Agricultural food production is greatly affected by postharvest diseases worldwide, such as the diseases caused by Alternaria species, which are very common in several crops. The management of fungal infections around the world largely relies on fungicides. In this context, the control of diseases such as early blight caused by Alternaria solani in potatoes and Alternaria linariae in tomatoes has mainly consisted of the application of fungicides, with negative impacts on the environment and human health. Recently, the application of ‘omics’ and gene editing through the CRISPR/Cas9 system and RNAi technologies demonstrated their effectiveness as emerging greener alternatives for controlling phytopathogenic fungi. Additionally, coatings based on essential oils and microbial antagonists suggest alternative strategies for controlling phytopathogenic fungi that are respectful of the environment. This review presents an exhaustive literature review focused on using greener alternatives to the traditional management of postharvest diseases associated with Alternaria species, such as inhibiting pathogenicity from their phytopathogenic genes using gene editing based on CRISPR/Cas9 and RNAi technologies. The review also presents coatings based on essential oils and microbial antagonists as greener strategies for Alternaria control. Biological processes of maximum efficiency can replace chemical methods for controlling phytopathogenic fungi, preserving healthy conditions in agricultural lands and ecosystems. This is possible with the rise of ‘omic’ technologies, the CRISPR/Cas9 tool, and RNAi technology. Greener control methods of Alternaria fungi can increase agricultural production, improving the economy and global health.
Microplastics (MPs) and nanoplastics (NPs) are plastic particles of concern worldwide as they negatively affect the environment and human health. The interactions of microorganisms with microplastics and nanoplastics are different. On one hand, microorganisms might biodegrade MPs/NPs through enzymes, but on the other hand, they serve as vehicles to spread diseases or negatively affect their viability. As a result, several studies have reported disturbances in soil balance and negative impacts on geochemical cycles. With an increasing number of investigations into microorganisms and their interactions with MPs and NPs, this study demonstrates a growing interest in biodegradable alternatives. A bibliometric analysis of 719 documents published from 2010 to December 2021 presents the research landscape on the interaction of microplastics and nanoplastics with microorganisms. The study shows that China, the United States, the United Kingdom, and Germany lead the scientific production on this topic. However, in situ studies of large-scale pilot applications in the environment are scarce. More research funding from governments in the form of national action that stimulates national and international cooperation through universities, institutes, and industries is required. There is an urgent need for studies focusing on the degradation mechanisms of various microorganisms through the characterization of enzymes involved in the chemical modification of these emerging contaminants (MPs/NPs) with transparent standard methodologies. Moreover, there is no standardization for MP/NP extraction or characterization methodologies for different environments, especially in atmosphere. The patent need for alternative pathways for MP/NP degradation is evident, using microorganisms such as bacteria, fungi, microalgae, and a consortium (peripheral biofilms) to mitigate the negative impact of the constantly increasing anthropogenic MP/NP pollutants in the world.
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