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Six isolates recovered from coffee seeds giving off a potato-like flavour were studied. Gene sequencing (rrs and rpoB) showed they belong to the genus Pantoea. By DNA-DNA hybridization, the isolates constituted a genomic species with less than 17 % relatedness to 96 strains representing enterobacterial species. Multilocus sequence analysis (gyrB, rpoB, atpD and infB genes) showed the isolates to represent a discrete species of the genus Pantoea. Nutritional versatility of the novel species was poor. The novel species is proposed as Pantoea coffeiphila sp. nov. and its type strain is Ca04 T (5CIP 110718 T 5DSM 28482 T ).

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Ochratoxin A (OTA) is a mycotoxin that contaminates several foodstuffs, including cocoa. It has nephrotoxic, teratogenic and carcinogenic properties in humans. The effect of post-harvest processing and storage on the OTA contamination of cocoa was studied over three successive cocoa seasons (2005, 2006 and 2007) in Cameroon. The type of fermentation (box or heap) did not significantly influence bean OTA content, which varied from undetectable (<0.03 ng/g) to 0.25 ng/g, remaining below 2 ng/g (the defined standard for cocoa beans). However, pod damage and late pod opening were aggravating factors for OTA contamination of cocoa. If pods were not intact (intentionally or naturally damaged), OTA was found in samples with contents of up to 75.5 ng/g before processing and 32.2 ng/g after 4 months' storage. This contamination exceeded the levels tolerated for export. In addition, some of the cocoa produced is processed locally and consumed as chocolate, cocoa powder, chocolate filled sweets, cocoa-based drinks and cocoa butter. In Cameroon, the average daily consumption of cocoa by-products is estimated at 0.75 g/d for adults and 1 g/d for children. Consequently, for maximum OTA contamination of cocoa beans, the maximum daily exposure to OTA would be 1.61 ng/kg bw/d in young children weighing around 20 kg, greatly contributing to the exposure of young consumers.