Nitric oxide (NO') is a physiological messenger formed by several cell types. Reaction with 02 forms oxides that nitrosate amines at pH values near 7. We now report experiments in which NO' was added to intact human cells and to aerobic solutions of DNA, RNA, guanine, or adenine. TK6 human lymphoblastoid cells were mutated 15-to 18-fold above background levels at both the HPRT and TK gene loci. Xan-
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