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SVXOPSIS. Parasites belonging to the genus Cytamoeba have been found in amphibians from central and northern California. The infected animals and their numbers were (URODELA) Aneides flavipunctatus (2), A. lugubris (29), Batrachoseps a. atttnuatus (81). Dicamptodon ensatus (2), and (SALIENTIA) Rana b. boyli (1). The rates of infection varied between 0.6% and 29.3%, and the number of parasites per infected cell ranged from one to seven. Preparations stained with haematoxylin and Romanowsky dyes indicated several types of parasite: some were homogeneous, others possessed several dark staining granules and some possessed large, dark staining central bodies or peripheral dark staining rims. Parasites were seen in the erythrocytes, the plasma and, occasionally, in endothelial cells and monocytes. They were found in the peripheral and heart blood and in the blood of the liver, spleen, kidneys and lungs. Cell division was common in erythrocytes, and yielded daughter cells of identical size. In some cases, chains of 3 to 5 individuals were seen and, rarely, three bodies, in a cloverleaf configuration, were produced as the result of a single division. Following acid hydrolysis, methylene blue‐stained parasites showed a peripheral ring of granules, and, in some, a few stained centrally located bodies.

SYNOPSIS. Cultures of Trypanosoma ranarum at room temperature, 20° or 25°C had similar patterns of growth and cyclic development, i.e., change from pear‐shaped to slender forms. At 9° the cycle of development and reproduction was compressed, but returned to normal after introduction to optimum temperatures. A compressed cyclic and reproduction rate was noted at 31° but return to optimum temperature did not reestablish normal cultural development. At 35° all flagellates were killed after 24 hr. The relation between reproductive capacity, cyclic development, and temperature is discussed speculatively.

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