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Cell cultures of trout gonad tissue (RTG-2) and Atlantic salmon heart, kidney, liver, and spleen tissue were inoculated with 50 50% tissue culture infective doses (TCID50) of infectious pancreatic necrosis (IPN) virus per cell, and the titer of cell-associated and released virus was determined from 2 to 16 h postinoculation (PI). Cover slips were collected over the same period and stained for IPN viral antigen by the direct immunofluorescent (FA) technique. Viral replication was detected after a latent period of approximately 2 to 4 h and reached a peak titer of 108-2 to 108 4 TCID6O per ml at 8 to 10 h PI. The release of virus was more rapid in Atlantic salmon cells than in RTG-2 cells. Viral antigen was first detected by FA from 3 to 4 h PI. Approximately 75 to 80% of the cells contained antigen in the cytoplasm 9 to 11 h PI. The direct FA technique was found to be a sensitive method for detecting IPN virus in infected cells. Three strains of IPN virus were tested for serological cross reactions by FA and virus neutralization tests. PI).

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Cancer is usually detected by visualization. Clinical biochemical parameters, eg, cancer profile (CA profile) and longevity profile (expanded CA profile), are capable of signaling a developing malignancy much earlier. The methods used in this study are chemiluminescence (HCG, CEA, TSH, DHEA-S), IRMA (HCG), and enzyme kinetics (PHI, GGTP). HCG, the pregnancy/malignancy hormone, is the autocrine proliferative factor. PHI is a neurokine, the autocrine motility factor. Telomerase is the immortality factor. According to this investigator, all 3 factors must be present for the development and sustenance of malignancies. Our data confirm the presence of PHI and HCG in most, if not all, cancers. Reports indicate telomerase in biopsy cancer specimens, and it was not detected in normal tissues. Studies are underway to establish the presence of the enzyme in human serum or plasma. Clinical laboratory results have confirmed positive CA profile tumor markers in approximately 90% of hundreds of pathologically established malignancies. Breast cancer yielded 92% positives, lung cancer 97%, and colon cancer 93% positive CA profiles. The clinical laboratory adoption of the herein proposed CA profile may not only warn of a developing, not yet diagnosed case, but it also may be useful for progress monitoring. The longevity profile is a conglomerate of laboratory tests for cancer, coronary risk factors, sex hormones, bone health, adrenal stress, and generally an overall examination of most of the organs. It is a biochemical full body scan without radiation. Category:Chemistry