Background Canine cloning technology based on somatic cell nuclear transfer (SCNT) combined with genome-editing tools such as CRISPR-Cas9 can be used to correct pathogenic mutations in purebred dogs or to generate animal models of disease. Results We constructed a CRISPR-Cas9 vector targeting canine DJ-1. Genome-edited canine fibroblasts were established using vector transfection and antibiotic selection. We performed canine SCNT using genome-edited fibroblasts and successfully generated two genome-edited dogs. Both genome-edited dogs had insertion-deletion mutations at the target locus, and DJ-1 expression was either downregulated or completely repressed. Conclusion SCNT successfully produced genome-edited dogs by using the CRISPR-Cas9 system for the first time.

Background: Canine cloning technology based on somatic cell nuclear transfer (SCNT) combined with genome editing tools, such as CRISPR/Cas9, can be used to correct pathogenic mutations in purebred dogs or to generate animal models of disease.Results: In this study, we constructed a CRISPR/Cas9 vector construct targeting the canine DJ-1 gene. Genome-edited canine fibroblasts were established by transfection of the vector following antibiotic selection. We performed canine SCNT using genome-edited fibroblasts and successfully produced two genome-edited dogs. Both genome-edited dogs had indel mutations at the target locus, and expression of the DJ-1 gene was downregulated or completely repressed.Conclusion: In conclusion, SCNT successfully produced genome-edited dogs using the CRISPR/Cas9 system for the first time.