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Objective: To investigate the regulative effect of microRNA-338-3p on colorectal carcinoma cell invasion and migration. Methods: The microRNA-338-3p expression pattern of colorectal carcinoma tissues and cell lines was detected by real-time reverse transcriptase polymerase chain reaction. The protein level of smoothened was detected by western blot analysis. Furthermore, colorectal carcinoma cells were pretreated with or without anti-smoothened-small interfering ribonucleic acid prior to the addition of pre-microRNA-338-3p or anti-microRNA-338-3p. The status of colorectal carcinoma cell invasion and that of migration were detected by transwell assay and wound healing assay, respectively. Results: The expression of microRNA-338-3p was significantly down-regulated in colorectal carcinoma tissues in comparison with those in the adjacent non-tumorous tissues, and the value was negatively related to advanced tumor, node, metastasis stage and local invasion. The expression of microRNA-338-3p in colorectal carcinoma cells transfected with premicroRNA-338-3p p was significantly increased. Furthermore, over-expression of microRNA-338-3p inhibited the expression of smoothened protein in colorectal carcinoma cells, which showed obviously suppressed invasion and migration ability. The expression of microRNA-338-3p in colorectal carcinoma cells transfected with anti-microRNA-338-3p was significantly decreased. Moreover, the down-regulated expression of microRNA-338-3p caused the upregulated expression of smoothened protein in colorectal carcinoma cells, which showed significantly enhanced invasion and migration ability. However, anti-smoothened-small interfering ribonucleic acid largely, but not completely, reversed the effects induced by blockage of microRNA-338-3p, suggesting that the regulative effect of microRNA-338-3p on colorectal carcinoma cell invasion and migration was indeed mediated by smoothened. Additionally, smoothened was identified as a direct target of microRNA-338-3p by luciferase assay. Conclusions: MicroRNA-338-3p could inhibit colorectal carcinoma cell invasion and migration by inhibiting smoothened expression.

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Abstract. To evaluate the color changes of tetracycline stained teeth after restored with IPS-EMPRESS Ⅱ ceramics. Simulated tetracycline stained teeth were divided according to staining degree into four groups: mild, moderate, severe, and very severe groups; each group had ten specimens which were repaired with IPS-EMPRESS Ⅱ ceramics veneers, bonded with Vitique Esthetic Cementation System. The ShadeEye-NCC color system was used to analyze the change in L, a and b. ΔE were calculated to compare color difference among the four groups.Before and after bonding, the values of L, a, b were significantly different (P <0.05). Mild tetracycline stained teeth bonded using resin adhesive without opaque bonding have a better effect than moderate and severe tetracycline stained teeth bonded with opaque resin adhesive; while, it is best to use other restorations to repair very severe tetracycline stained teeth. The IPS-EMPRESS Ⅱ ceramics can effectively improve the color of discolored tooth, however , the final color of the veneered teeth is easily influenced by the color of the abutment teeth. Aluting agent with color masking ability can do some improvements in the final color. An appropriate bonding agent should be chosen to perfect the final results of the veneered tooth.