The degradation of several Asian bamboo species by white-, brown-, and soft-rot fungi was investigated under laboratory conditions by means of different test methods. Severe deterioration was caused by all three fungi types. The bamboo species differed in durability. Samples from 6 months young culms decayed more than older ones. There were no significant differences between 1-and 3-year-old culms. Samples taken from the culm top were more vulnerable to decay than those from the bottom. Wet bamboo samples with soil contact were especially degraded by the white-rot fungus Schizophyllum commune, whereas the brown-rot fungus Coniophora puteana produced the greatest mass loss in drier samples. The sealing of bamboo crosscut ends reduced the rate of decay.
The aim of this research was to determine the chemical composition, antioxidant and antibacterial properties of the essential oils from Metaplexis japonica and isolation of antibacterial constituents from the essential oils. Results showed that 63 components were identified in essential oils. Phenylethyl alcohol (77.978%), a-terpineol (31.810%) and docosane (21.644%) were the most abundent constituents of flower oil, leaf oil and fruit oil, respectively. Based on bioactivity-guided fractionation, three active constituents were isolated and identified as phenylethyl alcohol, a-terpineol and b-linalool. Both flower oil and phenylethyl alcohol showed high antibacterial performance, with inhibition zone from 25 AE 0.5 to 11 AE 0.6 mm at highest concentration, and MIC values ranging from 0.125 to 2%. In both DPPH and ABTS assay, the oils showed moderate antioxidant activity. These results indicate potential efficacy of active constituents and essential oils of M. japonica to control food-borne pathogenic and spoilage bacteria.
Proposed is a method to investigate degradation of lignocelluloses by pure cultures of basidiomycetes using preserving jars with vermiculite as reservoir for water and nutrients. Bamboo samples of Gigantochloa atroviolacea and Phyllostachys pubescens and wood samples of Fagus sylvatica and Pinus sylvestris were inoculated with the brown-rot fungus Coniophora puteana and the white-rot fungus Schizophyllum commune. The fungi were cultured on vermiculite containing different amounts of tap water or malt extract solution. Mass loss of the bamboos after 32 weeks was low and did not show a remarkable influence of moisture content and nutrient addition. However, considerable degradation of Pinus sylvestris sapwood occurred by C. puteana whereby moisture and nutrients influenced aggressiveness.
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