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.Adult Tessaratoma papillosa (Drury) (Hemiptera: Pentatomidae) secreted volatiles from metathoracic glands (MTG). Behavior and olfactory responses of adult bugs to their MTG secretions were tested using a Y-tube olfactometer and an electroantennogram (EAG). The results indicated that males were obviously attracted by female secretions and repelled by male secretions. Secretions of adult males elicited the greatest response of male antennae, whereas the lower response of male antennae to MTG secretions of adult females was recorded. The four compounds, (E)-2-octenal, undecane, dodecane and tridecane were tested for the EAG activity and behavior response with male adults at different concentrations. In EAG assays, the antenna of male T. papillosa showed the highest sensitivity to (E)-2-octenal (10 μL /μL) and the second highest sensitivity to undecane (10 μL /μL), but a similar sensitivity to other stimuli. Unlike EAG results, males were attractive to (E)-2-octenal (10-2 and 10-1 μL /μL) and tridecane (10 μL /μL), and repelled by (E)-2-octenal (10-3, 1, 10 and 102 μL /μL) and tridecane (10-1 μL /μL) in the Y-tube olfactometer. Our data suggested that (E)-2-octenal and tridecane may be part of a complex pheromone system in T. papillosa.

Preliminary results of petri dish bioassay showed that n-hexane and ether extracts of body surfaces of female and male Tessaratoma papillosa (Drury) were significantly attractive to the opposite sex and the same sex respectively. EAG results also demonstrated that both two kinds of solvent extracts could induce the EAG responses of adult T. papillosa. Sixteen components were identified and isolated from the extracts of adult T. papillosa body surfaces by gas chromatograph-mass spectrometer (GC-MS), of which tridecane was the common component of females and males and had the highest relative amount. In addition to three kinds of straight-chain alkanes which were undecane, dodecane and tridecane, there were other components such as (Z)-2,2-dimethyl-3-hexene, 2-hexen-1-ol acetate, (E)-2-hexen-1-ol acetate, 2,3,3-trimethyl-1-butene and 1-tridecene and others.