Living organisms are generally composed of complex cellular processes which persist only within their native environments. To enhance our understanding of the biological processes lying within complex milieus, various techniques have been developed. Specifically, the emergence of super-resolution microscopy has generated a renaissance in cell biology by redefining the existing dogma towards nanoscale cell dynamics, single synaptic vesicles, and other complex bioprocesses by overcoming the diffraction-imposed resolution barrier that is associated with conventional microscopy techniques. Besides the typical technical reliance on the optical framework and computational algorithm, super-resolution imaging microscopy resorts largely to fluorescent materials with special photophysical properties, including fluorescent proteins, organic fluorophores and nanomaterials. In this tutorial review article, with the emphasis on cell biology, we summarize the recent developments in fluorescent materials being utilized in various super-resolution techniques with successful integration into bio-imaging applications. Fluorescent proteins (FP) applied in super-resolution microscopy will not be covered herein as it has already been well summarized; additionally, we demonstrate the breadth of opportunities offered from a future perspective.

Summary In this study, a free‐standing MoS2 nanofilm on a porous carbon cloth (MoS2@PCC) was prepared for application as an anode in Li‐ion batteries. Uniform, non‐aggregated MoS2@PCC electrodes were synthesized via facile electric‐wire‐explosion, dip‐coating, and thermal sulfidation processes. The phase and morphologies were controlled using a variety of explosion media that had different carbon contents. The dip‐coating of PCC into a colloidal solution prepared by underwater explosion of Mo metallic wire and the thermal sulfidation process provided higher uniformity of MoS2 nanoparticles with no particle aggregation. This facilitated the charge transfer and accommodation of volume expansion of Li‐active MoS2 upon cycling. Consequently, the free‐standing MoS2@PCC electrodes exhibited enhanced lithium reactivity, high rate capability, and cycle durability, compared with the conventional MoS2 nanoparticle electrode.

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