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Polysaccharides from Pleurotus eryngii exhibit a variety of biological activities. Here, we obtained a homogeneous branched β-1,6-glucan (APEP-A-b) from the fruiting bodies of P. eryngii and investigated its effect on immunity and gut microbiota. Our results showed that APEP-A-b significantly increases splenic lymphocyte proliferation, NK cell activity and phagocytic capacity of peritoneal cavity phagocytes. Furthermore, we found that the proportion of CD4+ and CD8+ T cells in lamina propria are significantly increased upon APEP-A-b treatment. Additionally, APEP-A-b supplementation demonstrated pronounced changes in microbiota reflected in promotion of relative abundances of species in the Lachnospiraceae and Rikenellaceae families. Consistently, APEP-A-b significantly increased the concentration of acetic and butyric acid in cecum contents. Overall, our results suggest that β-1,6-glucan from P. eryngii might enhance immunity by modulating microbiota. These results are important for the processing and product development of P. eryngii derived polysaccharides.

Recently, a growing literature pay attention to the green buildings, and most of them focuses on design, energy simulation, and post-occupancy evaluation but rarely involves the integration analysis of energy consumption, indoor environmental quality, and occupant satisfaction in the operational stage. In this paper, the authors propose a comprehensive quantitative study based on energy-environment-satisfaction (EES) and take a three-star green building in Shanghai as an example. Through the use of real-time monitoring data, the study analyses the distribution characteristics of the parameters of EES. Meanwhile, this study discusses the differences between operational energy consumption and design parameters and also quantifies the exponential relationship between per floor personnel density and building energy consumption. Moreover, combined with the user satisfaction survey, some improvements are suggested. Furthermore, the relationship between daily energy consumption and the environmental parameters of daily energy consumption, PM2.5, CO 2 , temperature, relative humidity, and illumination is fitted, and the results indicate that there is a multivariate linear relationship with a correlation of 0.876. Through the sensitivity analysis, we found that the relative humidity affects 1.4 times as much as CO 2 . Therefore, its control value is critical to reduce energy consumption in the operation of green buildings.

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