Objectives: This research aims to assess the base level of knowledge and attitude of Applied Medical Sciences Students regarding mpox and whether an educational intervention could improve their knowledge and attitude. Methods: A quasi-experimental research was used, involving 960 medical students from Applied Medical Sciences College at Prince Sattam Bin Abdulaziz University, KSA. They were recruited from the beginning of November 2022 till the mid of January 2023 based on the non-randomized sampling method. A standardized, anonymous, and closed-ended questionnaire was used, compromising three main sections: participants’ demographics, knowledge, and attitudes toward the mpox epidemic. Results: Total knowledge scores of the studied sample in the pretest phase were 45.43 ± 6.29 compared to 65.03 ± 2.93 in the post-test phase. Besides, total attitude scores were 48.62 ± 4.78 before program implementation, while after conducting the program reached 70.65 ± 5.13. There was a notable improvement in the total knowledge score of the sample studied after the intervention was implemented, particularly for neurological manifestations. Conclusion: After the program’s implementation, there was an obvious improvement in the medical students’ total knowledge and attitude scores concerning the mpox epidemic. It is necessary to initiate well-organized training initiatives for all other medical faculties, paramedics, and applied health institutions in Saudi Arabia.

Background: Phenylketonuria is an autosomal recessive condition in which phenylalanine accumulates in the blood and bodily fluids due to a lack of Phehydroxylase activity. The study aimed to evaluate the effect of instructional guidelines on mothers' knowledge, practice and lifestyle of their children with phenylketonuria. Design: A quasiexperimental research design was utilized to achieve the study's aim. Setting: The study was conducted at genetic counseling clinic in the basic health care unit at Sohag City affiliated to the Ministry of Health (MOH). Sample: A purposive sample included 100 mothers accompanied by their children with Phenylketonuria. Tools: Two tools were used: Tool I: Mothers' knowledge and reported practice regarding phenylketonuria: a structured interview questionnaire was utilized and Tool II: Children's lifestyle assessment questionnaire. Results: The study revealed that there was a statistically significant improvement in post-intervention mothers' knowledge and reported practices and their children's lifestyle with phenylketonuria than pre-intervention. A statistically significant correlation detected between compliance with the prescribed diet and growth problems. No statistically significant correlation found between compliance with the prescribed diet and sleep problems. Conclusion: The study concluded that the instructional guidelines were effective in improving mothers' knowledge, reported practice, and their children's lifestyle with phenylketonuria. Recommendations: Health education program for mothers should be provided to increase their knowledge and reported practices about phenylketonuria, as well as identify the importance of a prescribed diet for children with this condition.