Water needed for daily use is viable water that is clean and healthy. Due to limited access to clean water, people buy bottled water and refillable drinking water. It is necessary to do clinical research at the laboratory to determine whether the water condition is feasible or not for consumption. The purpose of this study was to determine whether or not there is E.coli bacteria in the refillable drinking water and bottled water. This study uses an experimental method. Samples were taken from 15 refillable drinking water depots in Kecamatan Rawalumbu Bekasi and 15 bottled water samples. The bacterias identification was made by planting the samples in Blood Agar media and EMB. The results showed four positive samples of E. coli bacteria (26.7%) in the refillable drinking water depot at Kecamatan Rawalumbu Bekasi and one positive sample of E. coli bacteria (6.7%) in bottled water. It was also found that other types of bacteria such as Coliform (6.7%), Pseudomonas sp. (40%), Klebsiella sp. (13.3%), Staphylococcus sp. (6.7%), and Streptobacillus Gram + (13.4%). Key words: Refillable Drinking Water, Bottled Water, Escherichia coli.