Flavonoids secreted by legumes play a major role as signal molecules for attracting compatible rhizobia. The aim of this study was to assess and understand the diversity of microsymbionts nodulating Bambara groundnut (Vigna subterranea L. Verdc.) landraces of different seedcoat colours using restriction fragment length polymorphism and phylogenetic analysis. Seedcoat pigmentation of landraces had effect on the diversity of microsymbionts of Bambara groundnut. Even when planted together in one hole, nodulating bradyrhizobia clustered differently. For example, 16S rDNA-RFLP typing of rhizobial samples TUTVSBLM.I, TUTVSCRM.I and TUTVSRDM.I originating respectively from Black, Cream and Red landraces that were co-planted in the same hole at Manga in the Sudano-sahelian savanna, as well as TUTVSCRK.I and TUTVSRDK.I respectively from Cream and Red landraces co-planted at Kpalisogu in the Guinea savanna, revealed different 16S rDNA- RFLP types. Phylogenetic analysis of 16S rDNA, glnII, recA and atpD sequences showed that Vigna subterranea was nodulated specifically by a diverse group of Bradyrhizobium species (e.g. Bradyrhizobium vignae, and a novel group of Bradyrhizobium spp.) in soils from Ghana and South Africa. The recA gene phylogeny showed incongruency with the other housekeeping genes, indicating the possibility of lateral gene transfer and/or recombination events. The grouping of isolates according to symbiotic gene (nifH and nodD) phylogenies revealed inter- and intra-specific symbiotic plasmid transfer and different evolutionary history. The results also showed that a cropping history and physico-chemical environment of soils increased bradyrhizobial diversity in Ghana and South Africa.

Field studies on the effect of differing seedcoat colour on plant growth and symbiotic performance of three Bambara groundnut landraces using the 15N natural abundance technique and ureide analysis in the xylem stream and petiole extracts revealed a consistent pattern in nodule function between ureide levels and shoot δ15N values, as well as between ureide levels and percent N derived from fixation at Gbalahi, Kpalisogu, and Manga. At those three sites, where shoot δ15N was low and percent N derived from fixation high, ureide concentrations in the xylem sap and petiole extracts were also high, indicating that the two techniques were comparatively quite robust in estimating N2 fixation at a higher precision in the test Bambara groundnut landraces. A comparison of the effect of differing seedcoat pigmentation on the concentration of phenolics released by imbibed seeds showed that the Black landrace contained higher levels of flavonoids and anthocyanins, followed by the Red, and lowest in the Cream landrace. The Black landrace also recorded much higher nodule number and nodule fresh weight per plant, greater shoot biomass, lower shoot δ15N values, greater N derived from fixation, higher symbiotic N contribution, and increased water-use efficiency across all the five study sites, followed by the Red landrace, which produced the highest grain yield. Black seedcoat pigmentation in Bambara groundnut is therefore a marker for increased nodulation, N2 fixation, and plant growth that can be tapped in breeding programs for developing high N2-fixing, water-use efficient grain legumes for use by farmers in Africa.

In the N 2 -fixing symbiosis, the choice of a symbiotic partner is largely influenced by the host plant, the rhizobial symbiont, as well as soil factors. Understanding the soil environment conducive for the survival and multiplication of root-nodule bacteria is critical for microbial ecology. In this study, we collected cowpea-nodules from acidic soils in Ghana and South Africa, and nodule DNA isolates were characterized using 16S–23S rRNA-RFLP, phylogenetic analysis of housekeeping and symbiotic genes, and bradyrhizobial community structure through canonical correspondence analysis (CCA). The CCA ordination plot results showed that arrow of soil pH was overlapping on CCA2 axis and was the most important to the ordination. The test nodule DNA isolates from Ghana were positively influenced by soil Zn, Na and K while nodule DNA isolates from South Africa were influenced by P. The amplified 16S–23S rRNA region yielded single polymorphic bands of varying lengths (573–1298 bp) that were grouped into 28 ITS types. The constructed ITS-dendrogram placed all the nodule DNA isolates in five major clusters at low cut-off of approx. 0.1 Jaccard’s similarity coefficient. The phylogenetic analysis of 16S rRNA and housekeeping genes ( glnII, gyrB, and atpD ) formed distinct Bradyrhizobium groups in the phylogenetic trees. It revealed the presence of highly diverse bradyrhizobia (i.e. Bradyrhizobium vignae , Bradyrhizobium elkanii , Bradyrhizobium iriomotense , Bradyrhizobium pachyrhizi , and Bradyrhizobium yuanmingense ) together with novel/unidentified bradyrhizobia in the acidic soils from Ghana and South Africa. Discrepancies noted in the phylogenies of some nodule DNA isolates could be attributed to horizontal gene transfer or recombination.

Soybean [Glycine max (L.) Merrill] is an important oilseed crop worldwide and it has recently become the crop of interest in Ghana. In this study, 142 soybean accessions were genotyped with 34 SSR markers and concurrently evaluated for five quantitative and two qualitative phenotypic traits. Twenty‐nine of the SSR markers were polymorphic with mean allele number of 5.3, polymorphic information content (PIC) of 0.51 and gene diversity of 0.55. Molecular analysis based on unweighted paired group arithmetic mean (UPGMA) clustering and principal coordinate analysis (PCoA) was similar in explaining the extent of diversity within the accessions. Structure analysis placed most of the accessions into two subpopulations with 18 (12.7%) as admixtures. Principal component analysis (PCA) based on phenotypic traits revealed two clusters. Both UPGMA clustering‐based SSR data and PCA from phenotypic data showed similar results. The assembled germplasm is genetically diverse with high variation in flowering and maturity period, and key yield components which could be exploited in developing superior varieties well adapted to Ghana and West Africa.

Despite its importance in providing income and food for smallholder farmers, fodder for livestock, and improving soil fertility through biological nitrogen fixation, groundnut yields are lowest on farmers’ fields in Sub-Saharan Africa due to biotic and abiotic constraints. Foliar fungal diseases account for over 80% reduction in groundnut productivity in some parts of Ghana. Unfortunately, chemical control of these foliar diseases has not yielded the desired results. Meanwhile, advances in phenotyping for disease tolerance in other crops have established a strong relationship between stay-green trait and foliar disease tolerance. However, this relationship has not been explored in groundnut. This study was designed to determine the genetic control of the stay-green trait and its relationship with leaf spot disease severity in groundnut. Twenty-five advanced groundnut breeding lines with varying degrees of tolerance for leaf spot tolerance were evaluated under diseased and disease-free conditions, after which four were selected for genetic studies. Results showed significant (p<0.001) differences among the genotypes for early leaf spot (ELS), late leaf spot (LLS), leaf area under greenness (LAUG), SPAD chlorophyll meter readings (SCMR), and yield traits. Leaf spot diseases caused 4.95 t·ha−1 (64.54%) pod yield reduction in CHINESE, the widely cultivated groundnut variety in Ghana. There was a strong correlation between LAUG and ELS (r = 0.82, p<0.001) and LLS (r = 0.63, p<0.001), and genotypes that were stay-green had tolerance to both diseases. Stay-green trait in groundnut was detected to be under the control of a single recessive gene and hence may be used to select for ELS and LLS resistance.