In the present study, Anabaena PCC550, Anabaena PCC574 and Cylindrospermum PCC518, Cylindrospermum PCC 567 have been subjected to 7 different inorganic culture media. In order to identify the best growth medium i.e.; optimized medium, the nutrient requirement of these two algae have been evaluated as prime requisite. The present investigation analyzed the growth of wet biomass of the four microalgae. In order to attain optimal growth of Anabaena and Cylindrospermum species, the 7 culture media employed in the current study were (i) BG11 medium (ii) Knoops medium (iii) Cyanophycean agar medium (iv) Modified Bristols medium (v) Prigsheims Medium (vi) Foggs Medium and (vii) Algal culture medium. Highest growth on 60th day was seen in Cylindrospermum PCC 518 (0.134mg/100ml), Anabaena PCC 574 (0123mg/100ml), Cylindrospermum PCC 567 (0.098mg/100ml), Anabaena PCC 550 (0.094mg/100ml) in Algal culture media which shows luxurient growth when compared to BG11, Foggs Media, Modified Bristols media, Knoops media. While, Prigsheims medium did not show any growth of Anabaena PCC550, Anabaena PCC574 and Cylindrospermum PCC518, Cylindrospermum PCC 567.