Entomopathogenic fungal isolates of Arachnid origin were assessed for their ability to produce mortality and inhibit egg hatching in Boophilus microplus with the aim of selecting an isolate for development into a myco-acaricide for control of cattle ticks. The ability of the most promising isolate to target developmental stages of more than one tick species and the optimum concentration of fungal inoculum to be used for future studies were determined. Metarhizium anisopliae was the most pathogenic of the three fungal species tested on B. microplus, producing shorter average survival times (ASTs) for engorged adults (AST = 5.2 +/- 0.1 days) and larvae (AST = 9.3 +/- 0.4 days), and a longer average hatching times (AHT; AHT = 19.8 +/- 0.5 days) in comparison to Simplicillium lamellicola and Paecilomyces farinosus. In comparative studies on two tick species with similar life cycles, M. anisopliae produced a shorter AST in engorged adult B. microplus (AST = 8.8 +/- 0.3 days) than Rhipicephalus sanguineus (AST = 10.3 +/- 0.3 days). M. anisopliae was pathogenic to larvae of B. microplus (AST = 7.7 +/- 0.4 days), however, had no effect on larvae of R. sanguineus (AST = 14.6 +/- 0.3 days) as the AST of this treatment was similar to its untreated control (AST = 14.1 +/- 0.4 days). M. anisopliae lengthened the AHTs in both B. microplus (AHT = 16.4 +/- 0.3 days) and R. sanguineus (AHT = 16.7 +/- 0.3 days) in comparison to the controls. The ASTs of engorged adult B. microplus treated with M. anisopliae shortened as the concentration was increased from 1 x 10(7) to 5 x 10(8) conidia/ mL. A further increase in concentration, 1 x 10(9) conidia/mL (AST = 10.2 +/- 0.4 days) did not shorten or lengthen the AST in comparison to 5 x 10(8) conidia/mL (AST = 9.4 +/- 0.3 days).
Since its accidental introduction into the island of Grenada in 1994, Maconellicoccus hirsutus [Homoptera : Pseudococcidae], commonly named the Hibiscus or Pink Mealybug (HMB), has been inexorably spreading through the Caribbean islands where it has become a major pest on several crops in 24 Caribbean Islands. This pest was also reported in Guyana threatening South and Central America. M. hirsutus is a very prolific pest that injects a toxin at the point of feeding, causing severe distortion of leaves, new shoots and fruit. Initial use of physical and chemical control methods were ineffective. In addition, because of its wide host range and its rapid geographie expansion, not only to agricultural land but also to home gardens and forest areas, biological control appeared as the most suitable method to manage the HMB populations. Three natural enemies were selected for this biocontrol effort: the predatory beetles Cryptolaemus montrouzieri Mulsant and Scymnuscoccivora Ramkrisna [Coleoptera : Coccinellidae] and the parasitoid Anagyruskamali Moursi [Hymenoptera : Encyrtidae]. A. kamali and C. montrouzieri were highly effective in bringing HMB populations under control. In newly infested countries, early introduction of biological control agents resulted in effective management of the pest. This experience provided a model for future management of other alien invasive pest species in the region.
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