A treatment program in a maximally supportive clinical environment can significantly reduce suicidal behavior in high-risk patients with bipolar I disorder.

For effects on milking performance, 12 Holstein cows were subjected to four stimulation routines: a) no stimulation, b) manual stimulation, c) manual stimulation with delayed milking, and d) intravenous infusion of .75 IU of oxytocin. In an added experiment, effects of the first two treatments on milking performance and release of oxytocin and prolactin were measured. Milk yield, fat, and protein content were not affected by any treatment. Machine-on times were shorter and peak and average milk flow rates higher for manually stimulated and oxytocin and prolactin in sera at resting were 5.25 microU/ml and 16.5 ng/ml. Differences between mean peak concentrations of oxytocin (16.6 and 16.0 microU/ml) and prolactin (28.1 and 27.5 ng/ml) for stimulated and unstimulated cows were small. Mean oxytocin concentration in stimulated cows peaked at 2 min compared with 5 min for unstimulated cows. No difference in time of prolactin release was detected. The timing of oxytocin release, rather than maximal concentration, could be the most important factor affecting milking.

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Membrane fractions from a lon strain of Escherichia coli but not a wild-type strain catalyze the incorporation of fucose from guanosine 5'-diphosphate-fucose into a lipid and into polymeric material. Both incorporation reactions specifically require only uridine 5'-diphosphate (UDP)-glucose. The sugar lipid was shown to be an intermediate in the synthesis of the polymer which was related to colanic acid. The sugar lipid had the structure (fucose3, glucose2)-glucose P-P-lipid. Its behavior on column and thin-layer chromatography, the rates of its hydrolysis in acid and base, and the response of its synthesis to inhibitors are all identical to the other sugar-lipid intermediates which have been shown to contain sugars attached to the C55-polyisoprenol, undecaprenol, by a pyrophosphate linkage. The membrane fractions from both the lon strain and the wild-type strain also catalyzed the incorporation of either glucose from UDP-glucose or galactose from UDP-galactose into a lipid fraction which was shown to contain the free sugar attached by a monophosphate linkage to an undecaprenol-like lipid. This lipid was isolated and its nuclear magnetic resonance spectra was identical to undecaprenol. The membrane fractions from both strains also incorporated glucose from UDP-glucose into glycogen and into a polymer that behaved like Escherichia coli lipopolysaccharide. Conditions were found where the incorporation of glucose could be directed specifically into each compound by adding the appropriate inhibitors.