Soil life supports the functioning and biodiversity of terrestrial ecosystems. Springtails (Collembola) are among the most abundant soil arthropods regulating soil fertility and flow of energy through above- and belowground food webs. However, the global distribution of springtail diversity and density, and how these relate to energy fluxes remains unknown. Here, using a global dataset representing 2470 sites, we estimate the total soil springtail biomass at 27.5 megatons carbon, which is threefold higher than wild terrestrial vertebrates, and record peak densities up to 2 million individuals per square meter in the tundra. Despite a 20-fold biomass difference between the tundra and the tropics, springtail energy use (community metabolism) remains similar across the latitudinal gradient, owing to the changes in temperature with latitude. Neither springtail density nor community metabolism is predicted by local species richness, which is high in the tropics, but comparably high in some temperate forests and even tundra. Changes in springtail activity may emerge from latitudinal gradients in temperature, predation and resource limitation in soil communities. Contrasting relationships of biomass, diversity and activity of springtail communities with temperature suggest that climate warming will alter fundamental soil biodiversity metrics in different directions, potentially restructuring terrestrial food webs and affecting soil functioning.

h i g h l i g h t s Mancozeb offer a risk to key-groups of non-target species in subtropical soils. F. candida and E. crypticus were the most sensitive speciesto Mancozeb. Tests with earthworms were insufficient to protect other non-target invertebrates populations. A multicriteria approach (soil types and organisms) is needed for pesticides risk assessment.

Soil life supports the functioning and biodiversity of terrestrial ecosystems1,2. Springtails (Collembola) are among the most abundant soil animals regulating soil fertility and flow of energy through above- and belowground food webs3-5. However, the global distribution of springtail diversity and density, and how these relate to energy fluxes remains unknown. Here, using a global dataset collected from 2,470 sites, we estimate total soil springtail biomass at 29 Mt carbon (threefold higher than wild terrestrial vertebrates6) and record peak densities up to 2 million individuals per m2 in the Arctic. Despite a 20-fold biomass difference between tundra and the tropics, springtail energy use (community metabolism) remains similar across the latitudinal gradient, owing to the increase in temperature. Neither springtail density nor community metabolism were predicted by local species richness, which was highest in the tropics, but comparably high in some temperate forests and even tundra. Changes in springtail activity may emerge from latitudinal gradients in temperature, predation7,8, and resource limitation7,9,10 in soil communities. Contrasting temperature responses of biomass, diversity and activity of springtail communities suggest that climate warming will alter fundamental soil biodiversity metrics in different directions, potentially restructuring terrestrial food webs and affecting major soil functions.

The biobed is a purification system, which reduces soil pollution for receiving pesticide residues from handling and washing machinery in agricultural areas. The aims of this study were (1) to assess ecotoxicity effects over time to soil fauna, posed by Lorsban ® 480 BR (Chlorpyrifos) and Dithane ® NT (Mancozeb) residues when disposed of in a biobed system compared with two subtropical soils, and (2) to assess ecotoxicity effects over time to soil fauna simulating an accidental spillage with Lorsban ® 480 BR at the biobed. A semi-field experiment was conducted for 420 days in southern Brazil, testing continuous disposal of washing pulverization tanks in biobeds, Typic Haploperox or Typic Hapludults. In addition, different biobeds received a single dose (1 L) of Lorsban ® 480 BR to simulate an accidental spillage. Chronic ecotoxicity tests were performed using Folsomia candida, Eisenia andrei, and Enchytraeus crypticus in different sampling times for both experiments. F. candida was the most sensitive species. The biobed system was able to eliminate effects from residues of both pesticides over time in all species, which did not happen in both natural soils. In accidental spillage simulation, even 420 days after contamination, F. candida did not show reproduction. The biobeds can be a feasible alternative for the disposal and treatment residues of pesticides, also for handling and washing pesticides activities. The system was efficient in promoting degradation and reducing ecotoxicity effects posed by Lorsban ® 480 BR and Dithane ® NT for soil fauna. It is a safe alternative to avoid soil contamination.

Brazil is considered a megadiverse country, but the soil fauna is still very poorly known. The aim of this study was to report, for the fi rst time, the abundance and genus composition of terrestrial enchytraeids (Enchytraeidae, Oligochaeta) in Savanna Tall Woodland (Cerradão) and a pasture in Cerrado Biome and in Upper Montane Atlantic Forest and a grassland in Atlantic Forest Biome. The enchytraeid density in Pasture and Cerradao was 2,036 and 18,844 (204 and 2,094, on average) individuals per square meter, respectively. At the Atlantic forest and Grassland, density was 9,666 and 12,242 individuals per square meter (1,075 and 1,471 on average). About genus composition for the studied areas, Enchytraeus and Hemienchytraeus were found in the four ecosystems evaluated, while Tupidrilus and Fridericia were found only in Cerradão and Atlantic Forest, respectively. Achaeta was absent in Upper Montane Atlantic Forest, but dominant in pasture, while Guaranidrilus was absent in Pasture, but predominant in the other ecosystems.