Esta revisão sistemática reuniu informações sobre a distribuição espacial no sul do Brasil do flebotomíneoMigonemyia migonei, um possível vetor de espécies de Leishmania causadoras da leishmaniose visceral(LV). Os artigos foram pesquisados nas bases de dados PubMed, Scielo, Web of Science e Scopus usandoas palavras-chave: “Migonemyia migonei AND Paraná”, “Migonemyia migonei AND Santa Catarina”,“Migonemyia migonei AND Rio Grande do Sul”, “phlebotomine AND Parana” , “flebotomíneo AND Paraná”e “flebotomíneo AND Paraná”, “flebotomíneo AND Santa Catarina;”, “flebotomíneo AND Santa Catarina”e “flebotomíneo AND Santa Catarina”, “flebotomíneo AND Rio Grande do Sul;”, “flebotomíneo AND RioGrande do Sul” e “sandfly AND Rio Grande do Sul”. A busca inicial identificou 322 artigos que atenderam aoscritérios de seleção. Em seguida, arquivos vazios ou duplicados foram excluídos. Os títulos foram triadose os textos completos foram obtidos. Esta revisão incluiu 36 artigos, abrangendo 72 dos 399 (18,04%)municípios do Paraná, um dos 295 (0,33%) de Santa Catarina, dois dos 497 (0,40%) do Rio Grande do Sul.Mg. migonei foi encontrado em 54 municípios do Paraná estado, em um município de Santa Catarina e emum do Rio Gr ande do Sul. Com base na ampla distribuição de Mg. migonei nos municípios paranaenses,é necessária maior atenção quanto aos casos de LV em humanos e animais, além de investigaçõesepidemiológicas desses casos de suspeita de autoctonia, bem como maiores esforços de prevenção econtrole. Mais estudos são necessários em Santa Catarina e Rio Grande do Sul.

Cryptosporidium and Giardia are the main etiologies of waterborne outbreaks caused by protozoa. These parasites are commonly detected in wastewater; however, there is little knowledge about the concentration of viable forms in treated sewage, mainly in small communities. To understand more about the presence of viable oocysts and cysts in domestic sewage, we monitored the affluent and effluent of a wastewater treatment plant (WWTP) in the inner city of Brazil. Ten samplings and seven follow-ups were performed in 2020. Samples were concentrated by centrifugation, filtration and purified by fluctuation. Viability was accessed by propidium-monoazide (PMA) associated with nPCR and qPCR. Both viable protozoa were detected in all raw sewage samples (average: 438.5 viable oocysts/L). Regarding treated sewage, Cryptosporidium was detected in all of the samples (average: 92.8 viable oocysts/L) and Giardia was detected in 70% with viable cysts in 30%. Considering the follow-ups, 31.17% of Cryptosporidium viable oocysts remained in the effluent after the treatment. High amounts of Cryptosporidium and a high frequency of Giardia were detected, therefore both arrived at WWTP and were discharged into the river. These alert the presence of agro-industrial effluents into domestic sewages and demonstrated the effectiveness of the concentration technique for monitoring protozoa in wastewater.

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