BackgroundLow cost unmanned aerial systems (UAS) have great potential for rapid proximal measurements of plants in agriculture. In the context of plant breeding and genetics, current approaches for phenotyping a large number of breeding lines under field conditions require substantial investments in time, cost, and labor. For field-based high-throughput phenotyping (HTP), UAS platforms can provide high-resolution measurements for small plot research, while enabling the rapid assessment of tens-of-thousands of field plots. The objective of this study was to complete a baseline assessment of the utility of UAS in assessment field trials as commonly implemented in wheat breeding programs. We developed a semi-automated image-processing pipeline to extract plot level data from UAS imagery. The image dataset was processed using a photogrammetric pipeline based on image orientation and radiometric calibration to produce orthomosaic images. We also examined the relationships between vegetation indices (VIs) extracted from high spatial resolution multispectral imagery collected with two different UAS systems (eBee Ag carrying MultiSpec 4C camera, and IRIS+ quadcopter carrying modified NIR Canon S100) and ground truth spectral data from hand-held spectroradiometer.ResultsWe found good correlation between the VIs obtained from UAS platforms and ground-truth measurements and observed high broad-sense heritability for VIs. We determined radiometric calibration methods developed for satellite imagery significantly improved the precision of VIs from the UAS. We observed VIs extracted from calibrated images of Canon S100 had a significantly higher correlation to the spectroradiometer (r = 0.76) than VIs from the MultiSpec 4C camera (r = 0.64). Their correlation to spectroradiometer readings was as high as or higher than repeated measurements with the spectroradiometer per se.ConclusionThe approaches described here for UAS imaging and extraction of proximal sensing data enable collection of HTP measurements on the scale and with the precision needed for powerful selection tools in plant breeding. Low-cost UAS platforms have great potential for use as a selection tool in plant breeding programs. In the scope of tools development, the pipeline developed in this study can be effectively employed for other UAS and also other crops planted in breeding nurseries.

Diarrheal disease is an important health challenge, accounting for the majority of childhood deaths globally. Climate change is expected to increase the global burden of diarrheal disease but little is known regarding climate drivers, particularly in Africa. Using health data from Botswana spanning a 30-year period (1974–2003), we evaluated monthly reports of diarrheal disease among patients presenting to Botswana health facilities and compared this to climatic variables. Diarrheal case incidence presents with a bimodal cyclical pattern with peaks in March (ANOVA p < 0.001) and October (ANOVA p < 0.001) in the wet and dry season, respectively. There is a strong positive autocorrelation (p < 0.001) in the number of reported diarrhea cases at the one-month lag level. Climatic variables (rainfall, minimum temperature, and vapor pressure) predicted seasonal diarrheal with a one-month lag in variables (p < 0.001). Diarrheal case incidence was highest in the dry season after accounting for other variables, exhibiting on average a 20% increase over the yearly mean (p < 0.001). Our analysis suggests that forecasted climate change increases in temperature and decreases in precipitation may increase dry season diarrheal disease incidence with hot, dry conditions starting earlier and lasting longer. Diarrheal disease incidence in the wet season is likely to decline. Our results identify significant health-climate interactions, highlighting the need for an escalated public health focus on controlling diarrheal disease in Botswana. Study findings have application to other arid countries in Africa where diarrheal disease is a persistent public health problem.

stricting their application in retrospective or validation studies (Hutchinson, 1991). Crop growth models require solar irradiance as input data, yetThe need for solar irradiance data for crop models there are few places where such data are routinely measured. For has led researchers to develop a number of methods for locations where measured values are not available, solar irradiance simulating such data. For example, some crop modelers can be estimated using empirical models such as the Bristow-(e.g., Rosenthal et al., 1989) have incorporated stochas-Campbell (B-C) model. This study was conducted to assess the spatial and seasonal accuracy of the B-C model for midcontinental locations tic weather generators into their simulations. These in Kansas. A 30-year data set from Manhattan, KS, was used to weather generators simulate irradiance and other metecalibrate and evaluate unmodified and modified forms of the B-C orological and climatological inputs based on probabilismodel. The effect of seasonality was investigated by subdividing the tic criteria. This approach eliminates the need for meayearly data into two subsets, a high noontime solar elevation angle sured solar irradiance; however, it seems reasonable period, ranging from DOY 121 to 273, and a low noontime elevation that estimated, rather than randomly generated, solar angle period comprising the remainder of the year. The B-C model irradiance values would also result in improved yield eswas also evaluated without seasonal division of the year. The calitimates. brated models were then tested against measured solar irradiance A number of techniques are available for estimating values for 10 sites distributed across the state of Kansas. Results solar irradiance. These vary in sophistication from simindicate that, for the calibration site at Manhattan, irradiance was ple empirical formulations based on common weather more accurately estimated using a modified form of the B-C model. For the yearly data, root mean square error (RMSE) was 3.9 MJ m Ϫ2 or climate data to complex radiative transfer schemes d Ϫ1 (25% error), compared with 5.2 MJ m Ϫ2 d Ϫ1 (24% error) for the that explicitly model the absorption and scattering of high solar elevation angle period and 3.6 MJ m Ϫ2 d Ϫ1 (32% error) the solar beam as it passes through the atmosphere. for the low solar elevation angle period. The RMSE for the 10 test Hall, Kansas State University, Manhattan, KS 66506-0801; R.L. Vanwhere A, B, and C are empirical coefficients. Although derlip,

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