The fatty acid compositions of brain phosphoglycerides from a freshwater fish, the rainbow trout (Salmo gairdneri), and a marine fish, the cod (Gadus morhua), were determined and compared with those from a terrestrial mammal, the rat. Fish brain lipids were characterized by a higher degree of unsaturation encompassing increased percentages of (n-3)PUFA (22∶6 and 20∶5) and lower percentages of (n-6)PUFA (20∶4 and 22∶4). However the distribution of fatty acids and specific PUFA between different phosphoglycerides was essentially similar in rat and fish brain tissue. PE and PS contained the highest percentages of 22∶6(n-3), PI was characterized by higher 18∶0 and 20∶4(n-6)/20∶5(n-3), and PC had higher 16∶0 and the lowest percentage of PUFA in all species. A generally similar pattern was found in the fish retinal phosphoglycerides except that PC was also rich in 22∶6(n-3). Overall trout brain phosphoglycerides were slightly more unsaturated than the cod lipids but with lower (n-3)/(n-6) ratios whereas cod retinal lipids were more unsaturated than the trout retinal lipids.