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I S S N 2348-6201 V o l u m e 6 N u m b e r 3 J o u r n a l o f A d v a n c e i n B i o t e c h n o l o g y | ABSTRACTChitosan is a bioactive amino polymer with wide applications. Mainly derived from chitin of marine sources, its traditional production still has some drawbacks such as irregular supply, low quality of product and lack of standardization. Farther, extraction processes are time-consuming with considerable environmental impacts, an extremely non-green process. Many works have shown the possibility of producingnative chitosan from Mucorales fungi, which is more easily extracted. Such process is advantageous due to low costs, process control and great possibility of high quality products. Moreover, the extraction of chitosan is faster and generates less pollutants. In this scenario, the possibility of standardized production allied with facilitated extraction and less probability of toxicological side effects from marine sources are characteristics that motivated this work. A Mucoralesisolate was cultured and the chitosans -native and semi-synthetic -obtained through heterogeneous extraction were compared. Results show substantial differences between them. Those differences are related to the processes required for extraction, yield, productivity, and quality. This work reinforces that Mucorales fungi excel as an alternative for chitosan production.