Perceptual experiences may arise from neuronal activity patterns in mammalian neocortex. We probed mouse neocortex during visual discrimination using a red-shifted channelrhodopsin (ChRmine, discovered through structure-guided genome mining) alongside multiplexed multiphoton-holography (MultiSLM), achieving control of individually specified neurons spanning large cortical volumes with millisecond precision. Stimulating a critical number of stimulus-orientation-selective neurons drove widespread recruitment of functionally related neurons, a process enhanced by (but not requiring) orientation-discrimination task learning. Optogenetic targeting of orientation-selective ensembles elicited correct behavioral discrimination. Cortical layer–specific dynamics were apparent, as emergent neuronal activity asymmetrically propagated from layer 2/3 to layer 5, and smaller layer 5 ensembles were as effective as larger layer 2/3 ensembles in eliciting orientation discrimination behavior. Population dynamics emerging after optogenetic stimulation both correctly predicted behavior and resembled natural internal representations of visual stimuli at cellular resolution over volumes of cortex.

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A 256 × 256 pixel spatial light modulator (SLM) is designed and constructed by the use of liquid-crystalon-silicon technology. The device is a binary electrically addressed SLM with a measured zero-order contrast ratio of 70:1 and an imaged contrast ratio of 10:1. The pixel pitch is 21.6 µm, which gives an array size of 5.53 mm. The electronic load time is 43 µs, and the 10%-90% switching time of the liquid crystal is ~75-80 µs at room temperature, which implies a maximum frame rate of ~8.3 kHz. We discuss the design trade-offs that are intrinsic to this type of device and describe how the primary application for the device in an optical correlator influenced the final design.

Polarization gratings (PGs) enable a novel architecture for dynamic non-mechanical steering of light over large angles and with large clear apertures. This beam steering approach has many applications in active sensing and optical communications. In this review, we describe some of the defining characteristics of this beam steering architecture and highlight several applications of the technology.

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