Background: Surgical site infections (SSI) constitute a major public health problem worldwide and are the second most frequently reported as Nosocomial infections. Surgical site infections are responsible for increasing the treatment cost, length of hospital stay and significant morbidity and mortality. Objectives: Bacteriological profile and antibiogram of Surgical Site Infection /Post-operative wound infection and to find drug useful for empirical treatment. Material and Methods: The retrospective study was conducted for a period of one year (January 2015 to Dec 2015) in the Department of Microbiology of BPSGMC Khanpur Kalan, Sonepat, Haryana. Samples were collected using sterile cotton swabs. 1687 patients clinically diagnosed of having SSIs were processed as per standard microbiological techniques. Antimicrobial susceptibility testing was done using modified Kirby-Bauer disc diffusion method. Results: Among 1687 patients screened for SSIs, 971 (57.5%) were culture positive. Staphylococcus aureus (25.5%) was the commonest organism followed by Escherichia coli (23.5%), Citrobacter species (17.3%) and Pseudomonas aeruginosa (9.9%). Antimicrobial profile of gram positive isolates revealed maximum sensitivity to Linezolid and Vancomycin, whereas among gram negative isolates Imipenem, Gentamicin, Piperacillin-Tazobactam, and Amikacin were found to be most sensitive. Conclusion: Staphylococcus aureus was the commonest pathogen isolated followed by E.coli (23.5%). Imipenem, Piperacillin/ Tazobactum, Gentamicin and Amikacin can be used as empirical treatment for gram negative bacilli and for gram positive organism Vancomycin and Linezolid can be used as empirical treatment in patients with Surgical Site Infection

Introduction: According to the estimates, nearly 70% of the disease burden occurs in Asia and is on constant rise. Dengue virus infection has emerged as a major public health concern across the globe in terms of mortality, morbidity and public health cost. Aims & Objectives: This study was undertaken to evaluate the significance of NS1 Ag detection in dengue IgM negative sera as marker for early detection and to compare the rapid detection test kits (RDT's) with Enzyme linked immunosorbent assay (ELISA). Material & Method: A total of 5283 samples from, were screened for dengue serology by Immunochromatography based lateral flow assay for presence of IgM and IgG anti Dengue antibody and NS 1 Ag. Results of 169 samples were further confirmed using ELISA technique for NS1 Ag in an attempt to compare RDT's with ELISA. Results: Out of 5283 samples screened, 146/5283 (2.76%) tested positive for dengue antibody, out of which 85/146 (58.21%) were positive for DEN Ig M and 61/146 (41.78%) showed presence of both IgG and IgM. Out of 5137 seronegative samples, 1930 (37.57%) were positive for DEN NS1 Ag. Overall, sensitivity of RDT was 100.00%, specificity-90.16%, PPV-94.73%, NPV-100.00 % and Efficiency of test-96.44%. Conclusion: NS1 Ag assay holds promise as useful tool in early diagnosis for detecting dengue infection during acute phase of infection when IgM antibodies are not formed to the detectable levels. Also, accurate and affordable diagnostic tests are a crucial component of combating this debilitating mosquito-borne infection.

Background: Hepatitis B virus (HBV) infects more than 300 million people worldwide and is common cause of liver disease and liver cancer. HBV, a member of the Hepadnaviridae family, is a small DNA virus with unusual features similar to retroviruses. HBV replicates through an RNA intermediate and can integrate into the host genome. The unique features of the HBV replication cycle confer a distinct ability of the virus to persist in infected cells. Aims & Objective: This study was designed to determine HBsAg Seropositivity among rural population attending inpatients and outpatients at tertiary care center with special consideration to childbearing age women. Material & Method: This retrospective study was conducted in the Department of Microbiology for three months in a tertiary care center catering rural population. A total of 1463 patients attending outdoor and indoor, were screened for HBsAg by lateral flow based rapid immunochromatographic card test (ICT) manufactured by J Mitra & Co Pvt Ltd. Results: Seropositivity of HBsAg was 41/1463(2.80%). Seropositivity was seen more in males 27/756(3.57%) as compared to females 14/707(1.98%). Age range 31-45 years was found to have highest prevalence rate with seropositivity of 15/279(5.38%). Among patients screened for HbsAg, we observed seropositivity of 2.37% (12/505) among women of childbearing age with age range 16 to 45 years. Conclusion: Findings in this study significantly contribute to the understanding of actual epidemiology of HBsAg in rural population especially women of childbearing age. Therefore, we should make efforts to prevent HBV infection by focusing on associated risk factors.