Pharmacokinetic interaction between ciprofloxacin, an antibacterial fluoroquinolone and flavonoid quercetin was investigated in the present study. Male wistar albino rats, weighing about 200-250 g were randomly divided into two groups, six rats in each group. Rats in group I (control) received ciprofloxacin alone (10 mg/kg PO). Group II received ciprofloxacin 30 min after pretreatment with quercetin (20 mg/kg PO). Rats were anaesthetized after ciprofloxacin administration for blood collection with a xylazine-ketamine combination. Blood samples were collected from jugular vein and tail clipping at predetermined time intervals prior to and at 0.33, 0.67,1, 1.5, 2, 4, 6, 8 and 12 h (20 , 40 , 6 0, 90, 12 0, 240 , 3 60, 48 0 a nd 720 min.) time interva ls after administration of ciprofloxacin. Peak plasma concentrations of ciprofloxacin will be reached in 1-2 h. after oral administration in rats. Plasma was separated by centrifuging at 3000 rpm for 10 min and stored at -20 o C until analyzed for ciprofloxacin by microbiological assay, using Escherichia coli (ATCC 25922). Based on plasma concentrations, the pharmacokinetic parameters were determined by non compa rtmenta l methods. Detectable plasma concentrations of ciprofloxacin in ra ts persisted upto 6 h when ciprofloxacin was given alone whereas ciprofloxacin was detectable up to 8 h in quercetin pretreated rats. Prior administration of quercetin modified the kinetic profile of ciprofloxacin as evidenced by the significantly (p<0.01) higher area under curve (AUC), area under the first moment curve (AUMC) and there was significant (p<0.01) decrease in V dss and Cl B when compared to untreated group.

Aim:-The experiment was carried out to study the influence of curcumin pre-treatment on pharmacokinetic disposition of ciprofloxacin following single oral administration in adult male wistar rats. Method:-Twelve rats were divided into two groups of six each .Animals in group-I were administered ciprofloxacin (10mg/kg body weight p.o), while animals in group-II received similar dose of ciprofloxacin after pre-treatment with curcumin (400 mg/kg body weight p.o). Rats were anaesthetized after ciprofloxacin administration by xylazine (10-20 mg.kg-1 IP) and ketamine (44-100 mg.kg-1 IP). Blood samples (200 μl) were collected into heparinised centrifuge tubes by tail vein clipping or jugular vein puncture as per convenience, prior to and at 0.33, 0.67,1, 1.5, 2, 4, 6, 8 and 12 h time intervals. Plasma concentrations of ciprofloxacin were determined by microbiological assay. Pharmacokinetic Analysis:-Plasma concentration versus time data of ciprofloxacin obtained in control and in pretreated group was utilized for calculating various pharmacokinetic parameters (β, t 1/2β , AUC 0-∞ , AUMC 0-∞, V d, Cl B, and MRT) by non compartmental methods (Gibaldi and Perrier, 1982) and computer software (PK Solver Version 2.0, 2010 by Zhang Yang). Statistical analysis:-All data were expressed as Mean±SEM. Differences in pharmacokinetic data between groups were analysed for statistical significance using unpaired student's't' test with Welch's correction using 'Instat' software Results:-Peak plasma drug concentration (C max), AUC 0-∞, elimination half life t½ β, Mean residence time (MRT) were significantly (p<0.05) longer in curcumin pretreated group. There is significant decrease in Steady state volume of distribution V dss andtotal body clearance Cl B in curcumin pretreated rats than in ciprofloxacin alone group. Conclusion:-The present study concluded that pretreatment with curcumin enhanced oral bioavailability of ciprofloxacin in rats.

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