The present study aims to develop and validate a simple, precise, accurate, stability indicating and isocratic reversephase high-performance liquid chromatography (RP-HPLC) method for estimating Bilastine in bulk and synthetic mixture. Bilastine from its degradation products were well separated and estimated on Discovery C8 column (250 mm x 4.6 mm, 5μm) using methanol: 0.1% ortho-phosphoric acid (55:45 %v/v) as a mobile phase and detection was performed at 276 nm by PDA detector. The degradation of Bilastine was studied under different ICH recommended stress conditions. The developed stability-indicating method was validated for system suitability, linearity, accuracy, precision, robustness, detection limit and quantitation as per ICH guidelines. The method was linear over 25-150 μg/ml. Mathematically computed Limit of Detection and Limit of Quantitation were found to be 0.19μg/ml and 0.57μg/ml, respectively. It was discovered that Bilastine degrades under acid and oxidation conditions. By using LC-MS/MS analysis, the structure of Bilastine breakdown products created amid acidic and oxidative settings was elucidated. A complete fragmentation pathway of Bilastine was established to elucidate the degradation products' structures using LC–MS/MS. The elemental concentrations of oxidation products and their fragmentation products were studied using the acquired mass values. The overall data was utilized to describe the degradation of products and their fragmentation process. The developed method can be used for routine analysis of Bilastine in dosage form as it can estimate of Bilastine in presence of excipients and degradation products.

Black pepper (Piper nigrum L.) is widely regarded as the "king of spices" due to its enormous trading share in the world market. This worldwide trade is not only attributed to its pungency properties, but widely due to its great health benefits and richness in the bioactive components. From the ancient times to the modern science, the black pepper has evolved as a one of the important medicinal agents which is not only used in the kitchen for its nutritional and heath properties, but widely employed in the pharmaceutical sciences for its scientifically proven pharmacological actions and therapeutic role. These actions are mainly due to the presence of the alkaloids, flavonoids, steroids, phenols, amides, lignans, essential oil etc. Piperine, a piperidine alkaloid and its derivatives are widely employed as a bioavailability enhancer and produces synergistic effects with many other phytoconstituents or medicinal drugs and contribute to improve the bioactivity, dose reduction, and even lessen the side effects of many pharmaceutical drugs. Black pepper is widely employed to increase appetite, treats the hypertension, cough, sinusitis, vomiting, epilepsy, gastric problems, dyspnea, worm infections, and skin diseases. It also found to possess several important pharmacological properties such as antimicrobial, analgesic, anti-inflammatory, antioxidant, anticonvulsant, hypolipidemic, hypoglycemic, hepatoprotective, neuroprotective, and anticancer activities. Due to these wide applications, the trade of black pepper is increasing day-by-day all the world. The black pepper is mainly produced throughout the world especially in Vietnam, India, Indonesia, Brazil, Malaysia, China, and Sri Lanka. In India, Karnataka, and Kerala account for almost 55 % and 34 % of the total production respectively. The present review outlined the recent advancements in therapeutic potential of black pepper (Piper nigrum L.) along with its synergism and analysed the trade performance in terms of area, production, import and export of black pepper in the world focusing on India.