The aim of this study is to examine phytochemical composition and evaluation of antimicrobial, antioxidant and cytotoxic activity of Anchusa officinalis plant extracts in different solutions: ethanol, chloroform, petroleum, acetone and ethyl acetate. A comparative analysis has shown that ethanol extract had the highest concentration of phenols (104.03 ± 0.63 mgGA/g), and the highest concentration of flavonoids (30.26 ± 0.40 mgRU/g). The highest concentration of the condensed tannins recorded in chloroform extract (74.65 ± 0.57 mg GA/g). The ethanol extract showed the strongest antioxidant and the best antimicrobial activity as compared to all other tested extracts, while the chloroform and the acetone extracts showed the best cytotoxic activity on cell line of mouse fibroblast carcinoma (L2OB). This is the first report of citotoxic activity of extract A. officinalis plant from Balkan region on tumor cell lines. HPLC analysis of A. officinalis plant extracts confirmed that the predominant polyphenol components were: rosmarinic acid, chlorogenic acid, naringenin, lutein-glycoside and rutin.

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The mineral composition of plant extracts from the Boraginaceae family was examined in this paper.The research includes the plants: Anchusa officinalis L., Echium vulgare L. and Echium italicum L.Content analysis of the elements was done using inductively coupled plasma with a massspectrophotometer. The results of the study showed that the extracts tested are rich in mineralcomposition, while the concentration of toxic elements is below the corresponding limit maximumpermitted concentration according to the recommendation of the World Health Organization.

Flavonols, phenolic acids and other polyphenols possess anti-inflammatory, antioxidative, antiproliferative, anticarcinogenic, antimutagenic, antibacterial or antiviral activities to greater or lesser extent (Liu et al. 2002;Ratnam et al. 2006). Cultivated strawberry (Fragaria × ananassa Duch.) has been known to have a high content of phenolic compounds and natural antioxidants (Aaby et al. Evaluation was performed of the effect made by integrated and conventional production systems on phenolic composition and antioxidant capacity of cv. Senga Sengana strawberry grown under greenhouse conditions over two consecutive years. The experiment also involved three different types of fertilizer. The integrated production system provides significantly larger quantities of polyphenols as well as a higher antioxidant capacity, compared to values obtained using the conventional system. The investigation into total phenolics content and total antioxidant capacity, as well as individual flavonols and phenolic acids (except gallic acid) content reveals a pronounced effect of the applied biofertilizers (PGPR 1 and PGPR 2). On the other hand, applied chemical fertilizer (Multi KMg) gives the best results in terms of anthocyanins content. These results indicate that there were significant differences in phenols content among integrated and conventional production systems, as well as between biofertilizers and a chemical fertilizer.

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