A BSTRACT Background: Dental caries is considered to be one of the most prevalent dental diseases among humans. It involves cycles of demineralization and remineralization. The etiology and pathogenesis of dental caries are multifactorial. The role of diet plays an important aspect in occurrence and progression of dental caries. Indians have differing dietary habits due to various religious and personal reasons. A substantial number of Indians are vegetarian by choice. Aim: The aim of this study was to assess the effect of vegetarian and mixed diet over the prevalence of dental caries. Materials and Methods: This observational cross-sectional study was conducted among 104 individuals. Healthy subjects who were 20–40 years of age, with no systemic disease, and not on any medications were included in this study. A thorough dental examination was performed by a single investigator with the help of mouth mirror and probe. The examination of patients involved the sequential assessment of teeth starting from 1 to 32. All the exposed and accessible surfaces of the teeth were examined for dental caries, and the data were recorded. Statistical Analysis: The data obtained were statistically analyzed using the Statistical Package for the Social Sciences (SPSS) software, version 11.0, and the results were tabulated. Univariate analysis was performed to find an association between caries and diet. Results: Distribution of the respondents according to dietary practices revealed 51% of the population consuming mixed diet and 49% consuming vegetarian diet. Oral hygiene was measured in terms of frequency of brushing tooth. Among the enrolled subjects, 57% brushed their teeth once a day and 42% brushed twice a day. No significant association was found between vegetarian and mixed dietary habits and dental caries. Two-sample independent t test was carried out to find the mean age. The mean age of caries group was 30.93 ± 12.27 and in without caries group, 27.83 ± 11.58, which is statistically not significant ( P = 0.218). Conclusion: The study did not show any significant association between dietary habits and prevalence of dental caries.