Background
The pharmaceutical industry and the National Regulatory Authorities are now focusing on the quantification of multi-component drugs for quality control testing.
Main body
Recently, the utilization of the ultraviolet spectrophotometric methods has become vital for the analytical studies for the routine analysis of different fixed-dose combination products either in the presence of a minor component in their combination or not. This article reviewed several published methods to those that have been applied to quantify some pharmaceutical combinations containing minor components through the last two decades.
Conclusion
The applied spectrophotometric methods are labor, time-saving, and also considered a cheap substitute for the overpriced high-performance liquid chromatographic technique.
Four spectrophotometric approaches were performed to determine a binary combination of Phenazone and Benzocaine in pure powder form and in pharmaceutical formations. This investigation submits the application of four techniques contingent on the presence of the extended area of the spectra of one compound in the binary mixture, these methods include Absorptivity Centering (a-centering), Absorbance Subtraction (AS), Amplitude Modulation (AM) and Concentration Value (CV). The linearity range for the above-mentioned approaches was found to be 3.0–15.0 μg/mL for Benzocaine in a-centering method and 3.0–30.0 μg/mL for Benzocaine and Phenazone in other advanced methods. The four techniques were evaluated as per ICH criteria and were successfully utilized for the determination of Phenazone and Benzocaine existing in pharmaceutical formulations. All results gained by the submitted approaches were statistically compared with a previously published method, and no important differences were detected.
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