Background and objectiveWork-related injuries (WRIs) are a major occupational health issue among healthcare workers (HCWs) worldwide. Unsafe work environments, including physical, chemical, and biological hazards, are significant contributing factors to WRIs. However, the prevalence of WRIs among HCWs in Jeddah, Saudi Arabia, and their associated risk factors remain largely unexplored. In light of this, this study aimed to investigate the prevalence of WRIs and associated risk factors among HCWs in Jeddah, Saudi Arabia. MethodsThis was an analytic cross-sectional study conducted at secondary hospitals of the Ministry of Health (MOH) in Jeddah by using a self-administered questionnaire to measure the prevalence of WRIs and their related factors. The Chi-squared test was used to compare variables. A p-value <0.05 was considered statistically significant. ResultsThe study involved 387 participants, of whom 283 (73.1%) were female. Most of the participants (n=226, 58.4%) agreed that personal protective equipment (PPE) was always available at their hospitals. Approximately two-thirds (n=251, 64.9%) agreed that they always used PPE. The overall prevalence of WRIs was 52%, with back injuries (32.6%), eye/mouth splashes (20.4%), and needle stick injuries (19.9%) being the most common. Years of work experience (p=0.014), type of profession (p<0.001), training in safety management (p=0.028), working hours (p=0.0001), working shifts (p=0.001), PPE availability (p=0.010), and sharp container availability (p=0.030) were significantly associated with WRIs. ConclusionThis study revealed a high prevalence of WRIs among HCWs in Jeddah, Saudi Arabia, with back injuries, eye/mouth splashes, and needle stick injuries being the most common types. The study also found that the injuries were significantly associated with the type of profession, experience, work hours, and shifts as well as the availability of safety management and equipment such as sharp containers and PPE.