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Multilevel confirmatory factor analysis was used to assess the factor structure of the six-factor student well-being scale at between- and within-class levels. A total of 2,707 Matthayom 4-6 students (equivalent to grades 10-12) from 71 classrooms (785 male and 1,922 female) completed this 54-items student well-being scale. Results showed that a single latent factor structure was acceptable fitted at between and within class levels. The reliability estimates of the six factors (Positive attitudes and emotions towards school, Enjoyment in school, Positive academic self-concept, (Absence of) Social problems in school, (Absence of) School worries, and (Absence of) Physical complaints) were 0.73, 0.38, 0.22, 0.13, 0.14, and 0.02, respectively, at the student level, whereas these estimates were 0.39, 0.44, 0.48, 0.61, 0.65, and 0.82, respectively, at the classroom level.