Context: Proper method of isolation plays a key role in the restoration of noncarious cervical lesions (NCCLs). Aims: The aim of this in vivo study was to compare the efficacy of two isolation techniques in restoring NCCLs using flowable composite. Settings and Design: Forty patients who fulfill the inclusion criteria were selected based on the power of the study (0.86). The participants were divided into two groups according to the split-mouth design. Materials and Methods: In Group A, lesion isolation was done using the Mylar matrix band with photocurable gingival barrier, and in Group B, using Metal matrix band and gingival barrier. The restorations were assessed immediately and after 6 months, using modified US Public Health Service criteria: marginal integrity, marginal discoloration, wear, retention, secondary caries, and postoperative sensitivity. Statistical Analysis: IBM SPSS (version 21.0) software was used. McNemar's and Chi-square tests were performed, considering P < 0.05 for statistical significance. Results: Both the groups demonstrated satisfactory clinical performance. Upon inter- and intragroup comparison of the two isolation methods, there was no statistically significant difference (P > 0.05). Conclusions: Within the limitations of the study, both the groups performed similarly in isolation of NCCLs. However, long-term clinical studies must be needed for further evaluation.

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