Alcohol-based hand sanitizers are being recommended as an infection prevention measure for COVID-19. Washing hands is a simple matter to eliminate and minimize germs on the hands with water and by adding certain ingredients, the use of hand sanitizer becomes must in recent circumstances. We have developed reliable and eco-friendly process for the synthesis of hand sanitizer gel that is a really useful tool in the fight against SARS-CoV-2, the virus that causes coronavirus disease 2019 (COVID-19). Considering scanty literature available on the use of custard apple peel and seed for preparation of hand sanitizer gel, present study was undertaken to evaluate effect of hand sanitizer from custard apple peel and seed extract. The antimicrobial activity of the formulated gel was tested against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis subsp. spizizenii, Salmonella typhimurinum and Shigella sonnei by The Quality Assurance and Testing Center 3 (QUATEST 3) in Ho Chi Minh city, Viet Nam.