This study was designed to investigate feasibility of canine ovariectomy using laparoscopy under local conditions of Pakistan, in comparison with conventional open surgery method. Twelve adult, clinically healthy bitches were randomly divided into equal groups. Animals of group A were subjected to laparoscopic ovariectomy, using three portal technique, while dogs of group B were ovariectomized through open surgery, using ventral midline approach. Operative time and amount of blood loss in dogs of both groups were recorded immediately after completion of operation, while scar length was measured at day 7 after operation, Moreover, cardinal and hematological variables were recorded for dogs of each group before surgery and then at day 1, 3 and 7 after operation. Results showed that operative time in bitches of group A was significantly higher (P<0.01) as compared to those of group B, whereas incision length and blood loss were higher in dogs of group B. However, there was no difference in cardinal variables between dogs of two groups before surgery and on day 1, 3 and 7 after operation. Among hematological variables, hemoglobin concentration and RBC count showed no variation between dogs of two groups and remained unchanged before and after surgery. Similarly, WBC count differed non-significantly between animals of two groups before and day 7 after surgery. However, it was higher in group B than group A on day 1 and 3 after surgery (P< 0.05). In conclusion ovariectomy through laparoscopy is an efficient procedure and can be recommended instead of open surgery in bitches.