The purpose of this study was to monitor concentration of selected biological and risk elements in testes and later epididymal spermatozoa motility of wild boars (Sus scrofa scrofa) as well as their association. Wild boars were hunted in region Žuhračka - Levice branch plant, Slovak Republic. Testes were surgically removed post-mortem and were transported to the laboratory. Spermatozoa were obtained by dissecting the distal portion of the cauda epididymis and were analysed by Computer assisted semen analyzer (CASA) system. Concentration of elements were measured by inductively-coupled plasma optical emission spectrometry (ICP-OES) and by cold vapor absorption spectrometer (MA-3 Solo Mercury Analyzer). Total spermatozoa motility was at the level of 44.29% and progressive motility was 18.47%. Concentration of elements in testes was in following order: K > Na > Mg > Ca > Fe > Zn > Al > Cu > Se > Mn > As > Cr > Pb > Mo > Sr > Ni > Ba > Cd > Li > Hg. Negative association was observed between Se and motility, progressive motility, further between As and velocity curved line and beat cross frequency. Mercury showed positive correlation with beat cross frequency. Our results suggest that some chemical elements accumulated from polluted environment can affect reproduction of wild animals.
The aim of the study was to determine the effect of training process on changes of quantitative and qualitative indicators of the movement mechanics of sport horses. A load controller was used to evaluate the motion mechanics. The training load was divided into three stages with different lengths, speeds and degrees of load. Tested animals were 14 horses of Slovak Warmblood breed. Our results have shown that gradual increase of load in the tested horses resulted in better spaciousness of movement, which was reflected in the elongation of the horse´s stride, regular movement and significant energy of the pelvic limbs (p <0.05). From our findings we can state that the effect of gender did not have significant effect on the number and length of steps in each degree of load. We have noticed a significant difference (p <0.05) in the "number of steps" parameter between the age categories of horses in the age up to 7 years (3.460 ±619) and the category aged up to 14 years (3.383 ±572). Gradual increase of the training load led to the economization of the movement and the long-term training process sled to the adaptation of the organism to repeated load.
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