Cincalok is a typical food from West Kalimantan made from fermented rebon shrimp containing astaxanthin, the most potent antioxidant in nature. This study investigated an efficient method for extracting astaxanthin from the cincalok using vegetable oils as solvents. Olive, sesame, grape seeds, coconut, and virgin coconut oil were used as alternative solvents. The effect of various parameters on extraction yield was also studied. N-hexane and acetone were also used for comparison. Amplitude level and extraction time were the factors investigated concerning extraction yield. Comparative studies between traditional extraction methods and extraction assisted by ultrasonication have also been carried out. The astaxanthin content as total carotenoids in oil extract was analyzed using a UV-vis spectrophotometer with a standard external method. The optimum ultrasound-assisted extraction condition of astaxanthin from cincalok was 40% amplitude for 3 minutes, with 100.62 μg/g of astaxanthin extraction yield when used virgin coconut oil as a solvent. In this way, oils enriched with astaxanthin are produced.
Shrimp species have been reported to contain astaxanthin, which has high antioxidant activities. They also contain omega-3 in the form of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), which can act as photoprotective agents that maintain healthy skin from reactive oxygen species (ROS) due to exposure to UV rays. In addition, fermentation has become an essential pre-treatment to extract the bioactive components in shrimp more easily. This study aims to extract oil from cincalok, a traditional Indonesian (especially in West Kalimantan) food made from Acetes shrimp fermented for 7–15 days. Cincalok oil was added to the lotion as a bioactive additive and sunscreen. Cincalok oil was extracted by the soxhletation method using n-hexane as solvent. The oil was then analyzed for its physicochemical properties, including density, viscosity, possible heavy metal contamination, and the profile of the fatty acids contained. The yield of cincalok oil extraction was 1.09 ± 0.05%, with the highest fatty acid content of 21.70% palmitic acid, 10.99% DHA, and 10.33% EPA. Cincalok oil also contains astaxanthin of 0.38 ± 0.02 mg/L oil. It has a viscosity of 69.71 ± 0.12 cP with a density of 0.93 ± 0.03 g/cm3. The analysis data of ICP-AES shows that there is no heavy metal contamination. The SPF value produced from cincalok oil lotion at 5 and 10% variations of cincalok oil was 15.17 ± 0.09 and 30.28 ± 0.49, respectively. The SPF value of lotion with the addition of cincalok oil was much greater than that of the base lotion, which was 2.16 ± 0.12.
Kerang Ale-ale (Meretrix Sp.) adalah salah satu komoditas perairan laut yang dimanfaatkan sebagai bahan pangan dan secara turun temurun dipercaya memiliki khasiat untuk pengobatan oleh masyarakat khususnya yang berdomisili di Kabupaten Ketapang, Kalimantan Barat. Tujuan penelitian ini adalah untuk mengetahui komponen bioaktif dan aktivitas antioksidan dari ekstrak kerang Ale-ale (Meretrix Sp.). Komponen bioaktif dianalisis secara kualitatif dengan pengujian fitokimia dan penentuan aktivitas antioksidan dengan metode scavenging radikal bebas dari DPPH. Hasil uji komponen bioaktif dari ekstrak kerang Ale-ale mengandung komponen antara lain alkaloid, flavonoid, saponin dan steroid. Berdasarkan hasil uji antioksidan, menunjukkan bahwa ekstrak metanol dari kerang Ale-ale memiliki aktivitas antioksidan kuat dengan nilai IC50 terendah 84,46 ppm.
Salah satu faktor kesuburan pada hutan mangrove adalah serasah daun yang mengalami proses dekomposisi. Proses dekomposisi serasah daun mangrove diuraikan sebagian besar oleh aktivitas bakteri. Adanya aktivitas bakteri pendekomposer dapat memperlaju proses dekomposisi serasah. Tingginya laju dekomposisi serasah daun mangrove berkaitan dengan degradasi kandungan utama serasah dan daur hara. Tujuan dilakukan penelitian ini adalah untuk mengetahui bakteri pendekomposer dan menentukan laju dekomposisi serasah daun Avicennia lanata yang terdapat di kawasan hutan mangrove Desa Sungai Bakau Kecil. Pengambilan sampel serasah daun dilakukan dengan metode purposive sampling dengan 2 lokasi pengamatan. Hasil penelitian diperoleh genus bakteri yaitu Bacillus, Kurthia, Sporosarcina, Listeria, Corynebacterium, Azotobacter, Alcaligenes, Pseudomonas, Serratia, Vibrio, dan Actinobacillus. Laju dekomposisi lokasi I mencapai 0,206 g/hari dengan persentase penyusutan mencapai 66,9 % dan lokasi II mencapai 0,19 g/hari dengan persentase penyusutan mencapai 63,7 % pada hari ke 60.
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