Restricted (non-systemic) inoculation of cucurbits, green bean, tobacco, and other plants with certain viruses, bacteria, or fungi has been shown to induce persistent, systemic resistance to a wide range of diseases caused by diverse pathogens. The non-specificity of this response has fueled speculation that it may also affect plant suitability for arthropod herbivores, and there is limited evidence, mainly from work with tobacco, which suggests that this may indeed occur. Young cucumber plants were immunized by restricted infection of a lower leaf with tobacco necrosis virus (TNV), and upper leaves were later challenged with anthracnose fungus, Colletotrichum lagenarium, to confirm induction of systemic resistance to a different pathogen. The response of arthropod herbivores was simultaneously measured on non-infected, systemically protected leaves of the same plants. As has been reported before, immunization with TNV gave a high degree of protection from C. lagenarium, reducing the number of lesions and the area of fungal necrosis by 65-93%. However, there was no systemic effect on population growth of twospotted spider mites, Tetranychus urticae Koch, on upper leaves, nor did restricted TNV infection of leaf tissue on one side of the mid-vein systemically affect mite performance on the opposite, virus-free side of the leaf. Similarly, there were no effects on growth rate, pupal weight, or survival when fall armyworm larvae were reared on systemically protected leaves from induced plants. In free-choice tests, greenhouse whiteflies oviposited indiscriminately on induced and control plants. Feeding preference of fall armyworms was variable, but striped cucumber beetles consistently fed more on induced than on control plants. There was no increase in levels of cucurbitacins, however, in systemically-protected foliage of induced plants. These findings indicate that pathogen-activated induced resistance of cucumber is unlikely to provide significant protection from herbivory. The mechanisms and specificity of induced resistance in cucurbits apparently differ in response to induction by pathogens or herbivores.
Scale insects (Hemiptera: Sternorryncha: Coccomorpha) are a very important insect group containing numerous pests of woody and herbaceous plants worldwide. The only complete study of any scale insects in Indonesia was by D.J. Williams on the mealybugs (Pseudococcidae sensu lato), published in 2004; the other scale insect families have only been documented in various small publications. Here we provide a complete checklist of the scale insects of Indonesia, which now contains 364 species belonging to 136 genera in 16 families. The family Diaspididae is the most diverse, with 158 species belonging to 44 genera, followed by Pseudococcidae with 105 species belonging to 32 genera, and Coccidae with 65 species belonging to 22 genera. The other families are, in order of size: Monophlebidae (26 species belonging to 9 genera), Rhizoecidae (12 species belonging to 4 genera), Asterolecaniidae (11 species belonging to 5 genera), Leconodiaspidae (7 species belonging to 3 genera), Cerococcidae (5 species belonging to 3 genera), Xenococcidae (5 species belonging to 2 genera), Ortheziidae (4 species belonging to 3 genera), Eriococcidae (4 species belonging to 2 genera), Aclerdidae (2 species belonging to 1 genus), and Kermesidae (1 species belonging to 1 genus).
Laboratory Evaluation of Local Isolates of Beauveria bassiana and Metarhizium anisopliae against Coffee Berry Borer, Hyphotenemus hampei, using spryaing method. Beauveria bassiana Bals (Vuillemin) and Metarhizium anisopliae (Metschnikoff) Sorokin are two species of fungi that have been studied widely and used as biological control of various insect pests, including coffee berry borer (CBB), Hyphotenemus hampei Ferrari. Laboratory studies were done in June – July 2017, to evaluate local isolates of both fungi species against CBB, the most destructive insect pest of coffee worldwide, including in Indonesia. B. bassiana was isolated from dead sweet potato weevil (Cylas formicarius) and green stink bug (Nezara viridula), and from soil. M. anisopliae was isolated from soil. The pure cultures of the fungi were mass-cultured on corn based media. The conidia harvested from 4 week colonies was hand sprayed directly upon CBB adults (females) and damaged coffee berry at the concentration of 109 ml-1. In additions, the conidia of B. bassiana isolated from C. formicarius alone was diluted to 106- 109 ml-1 and applied to damaged coffee berry. The results showed that when the conidia were applied directly upon the insects, the mortality of beetle were higher in B. bassiana than in M. anisopliae treatments. Pooled data indicated that B. bassiana isolated from death insects significantly caused higher mortality than did M. anisopliae isolated from soil. Beetle mortality was 76.7 and 80.0% for B. basiana, and 60.3 and 60% for M. anysopliae treatments. Application upon damage coffee berry indicated much lower mortality. Application of higher concentration upon damaged coffee berry resulted higher mortality, but data are not consistent. The mortality of CBB larvae was much less and negligible even at the highest conidia concentration.
Soil ecosystems are ecosystems related to the degradation of organic matter. University of Bengkulu is an area that has two kinds of soil ecosystems; secondary forest ecosystems and park ecosystems. Arthropods are one of the faunas living in the University of Bengkulu campus ecosystem. Camponotus dominates the secondary forest area, while Solenopsis dominates the park ecosystem. Data were obtained from two ecosystems (secondary forest ecosystem and park ecosystem). The results show that there are five classes with 199 individuals, while in the park ecosystem, there are four classes with 250 individuals.The secondary forest ecosystem diversity index value is 2,73, and the ecosystem diversity index value is 1,91. The evenness index value of the secondary forest ecosystem is 0,78, and the park ecosystem is 0,76. The secondary forest ecosystem dominance index value is 0,09, and the park ecosystem dominance index is 0,17. The diversity of soil arthropods in secondary forest and park ecosystems is in the category of moderate diversity. The even distribution of soil arthropods in both ecosystems is high. Moreover, there is no dominance by one type of soil arthropods in both ecosystems. The diversity of arthropods in the secondary forest ecosystem is higher than that of the park ecosystem. The number of individuals in the park ecosystem is higher than in the secondary forest ecosystem. In both ecosystems, the most commonly found are from the Formicidae family
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