Kinetic of reassociation of short DNA fragments were measured in eight ground squirrel species: Citellus undulatus, C. parryL C. relictus, C. dauricus, C. citellus, C. pygmaeus, C. fulvus and C. major. It was shown that 30-50% of their genome were represented by repeated sequences forming three kinetic fractions, i,e., very fast(Cot< 10 3), fast(Cot 10 -3 -3 × I0 -l) and intermediate (Cot 6 × 10 -1 -6 × 101). Based on parameters of DNA reassociation kinetics genome sizes of Citellus were estimated to range from 2.7 pg (C. dauricus) to 3.9 pg (C. pygmaeus and C.fulvus). Variation in genome sizes involves both the repeated and the non-repeated sequence components to approximately equal extents in all the species except C. dauricus. The linear quantitative relation between C-banding heterochromatin and both very fast and fast reassociated DNA fractions was established, but no connection with the intermediate fraction was found. No distinct relation was revealed between parameters of DNA reassociation kinetics and taxonomic status of species within genus or with the chromosome number of the karyotype.