BACKGROUND: The cumulating long-term changes in stable macromolecules, including non-enzymatic glycosylation of proteins and oxidative stress, are main fundamental reasons for development of late complications in patients with diabetes mellitus type II. OBJECTIVE: To evaluate some high-sensitive and specific proteins as early diagnostic tool and prognostic biomarkers of late complications of diabetes mellitus type 2 (T2DM). METHODS: Proteomic studies of blood plasma in patients with T2DM were performed through one-and two-dimensional electrophoresis of proteins in polyacrylamide gel, in the presence of sodium dodecyl sulfate-according to Laemmli (SDS PAGE). RESULTS: The spectrum of 70 proteins was proposed as tools in clinical/laboratory diagnostics and as independent prognostic biomarkers-predicting early development of disease complications. CONCLUSIONS: The identified proteomic biomarkers in blood plasma of patients with T2DM could serve as drug targets for early prevention and successful treatment of disease complications.