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Two groups were distinguished among patients with autoimmune neutropenia with manifest antigranulocytic antibodies. One group consisted of patients whose sera reacted with all forms of leukocytes (granulocytes, monocytes, T and B lymphocytes), like CD45 monoclonal antibodies. Sera of patients from the other group reacted only with polymorphonuclear leukocytes and monocytes, similarly as CD13 monoclonal antibodies. Immunologically distinguished types of neutropenia were characterized by a typical chronic or acute clinical course. Key Words: cluster of differentiation antigens; antigranulocytic antibodies; immune neutropeniasImmune neutropenias are little studied serious diseases. Antineutrophilic antibodies detected in patients with neutropenia have not been characterized in sufficient detail [4,5,10]. Recent studies with monoclonal antibodies (Table 1) MATERIALS AND METHODSSera of 15 patients with immune neutropenia aged 1-61 years were examined. Clinically the disease was characterized by acute or chronic neutropenia with or without concomitant bacterial or viral infections or local inflammations. Sera of all patients contained granulocytotoxic antibodies. In the majority of sera these antibodies were detected in titers 1:16-1:128. In three patients the titer of antibodies was no higher than 1:8.Complement-dependent cytotoxic test was the principal method of investigation [8,10]. Serum (1 lal) was pipetted into 60-weU plates under Vaseline oil, an equal volume of suspension with cell concentration of 3000-4000 eells/mm 3 was added, and the mixture was incubated for 1 h with polymorphonuclear leukocytes and monocytes at 4~ and with B and T lymphocytes at 18"C. Then 5 ~tl complement (fresh or lyophilized rabbit serum) was added into each well.The plates were incubated for 1.5 h at ambient temperature, after which the contents of the wells was then discarded and 1 rtl 1% Trypan Blue was added in each well. After 5 rain, the reaction was assessed by counting dead and live ceils. The result was considered positive if at least 50% cells were dead (2 points); no more than 10% cells were dead in control wells with nonimmune AB(IV) serum (0 points).

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