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Link to publicationCitation for published version (APA): Megens, R. T. A., Reitsma, S., Prinzen, L., Oude Egbrink, M. G. A., Engels, W., Leenders, P. J. A., ... Zandvoort, van, M. (2010). In vivo high-resolution structural imaging of large arteries in small rodents using twophoton laser scanning microscopy. Journal of Biomedical Optics, 15(1), 011108-1/10. DOI: 10.1117/1.3281672
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Take down policyIf you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. Abstract. In vivo ͑molecular͒ imaging of the vessel wall of large arteries at subcellular resolution is crucial for unraveling vascular pathophysiology. We previously showed the applicability of two-photon laser scanning microscopy ͑TPLSM͒ in mounted arteries ex vivo. However, in vivo TPLSM has thus far suffered from in-frame and betweenframe motion artifacts due to arterial movement with cardiac and respiratory activity. Now, motion artifacts are suppressed by accelerated image acquisition triggered on cardiac and respiratory activity. In vivo TPLSM is performed on rat renal and mouse carotid arteries, both surgically exposed and labeled fluorescently ͑cell nuclei, elastin, and collagen͒. The use of short acquisition times consistently limit in-frame motion artifacts. Additionally, triggered imaging reduces between-frame artifacts. Indeed, structures in the vessel wall ͑cell nuclei, elastic laminae͒ can be imaged at subcellular resolution. In mechanically damaged carotid arteries, even the subendothelial collagen sheet ͑ϳ1 m͒ is visualized using collagentargeted quantum dots. We demonstrate stable in vivo imaging of large...