Fistulated Holstein steers were used to deter-the highest count for holotrichs coinciding mine the. diurnal-pattern of rumen fermenta-with tlie trigh"ri "o"i""tiation of soluble cartron activity, soluble carbolrYdrate concen-bohydrates.-The diurnal pattern of total untration, p-rotozoa count and the concentration saturated tutty n.iai *tiri steers received the of long-chain fatty acids in rumen fluid when basal f corn oil Oi"t *u. inverse to that of 99ry-oil' tallow or bromochloromethane the baial f tal1ow diet. The occurrence of (P-CM) were included in the diet. There were comparable' concentrations of unsaturated fat differences (P. < 0.05) between diets in fer-in the rumen aig.rl;; b;i ar difierent times mentatlon-action (control + BCM ) basal before and after-feeding, suggests that both * lattolv,> control ) basal + corn oil diet). fals a{Iecr merhane suppiession by a similar ,lne soluble.carbohydrate concenlration fol-mechanism but at difl-erent stages of ferlowed a_ srmrlar pattern. Diet did not aflect mentation activily. The uplake of soluble car_ the tolal protozoa count. but inffuenced the bohydrate by holotrich protozoa appears to diurnal pattern. The holotrich,protozoa count have a rold in reducin'g t6"-q""'riiiv f". was converse to that of the oligotrich group, mented by bacteria to produce methane.
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