This study was carried out to illustrate multi-drug resistant Acinetobacter baumannii which have carbapenem resistance genes and to show its effect on mice hepatorenal functions. Forty four (4.2%) A.baumannii strains were isolated from 1050 positive cultures of all nosocomial pathogens isolated from Mansoura University Hospitals over one year. Sensitivity rates of A.baumannii were (28%) to imipinem, (66%) to meropenem, and (50%) to amikacin. Phenotypic detection of extended-spectrum ß-lactamases (ESBLs) was done by the Modified Hodge test which showed that (54.55 %) of A.baumannii were positive and 45.45% were negative. The bla OXA genes were identified in A.baumannii by PCR analysis of which (72.72%) were positive for bla gene and (29.54%) were positive for bla