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Molecular Rearrangement of Some Sterols 137 tionation. Rehydrogenation and refractionation of the tetradecahydrophenanthrene gave a product, b. p. 155-157°( 27 mm.), having re26d of 1.5003 which agrees with the value given by Pinkney and Marvel.6 In the fractionation of the reaction mixtures there were intermediate fractions which are not reported in the table. Their amounts in most cases represented only a few per cent, of the weight of phenanthrene originally used. However, with copper-chromium oxide at temperatures of 200-300°these unidentified products amounted to about 30% of the weight of phenanthrene submitted to hydrogenation. SummaryMethods have been given for the preparation of 9,

Lipofection, the transfer of genetic material into cells by means of cationic lipids, is of growing interest for in vitro and in vivo approaches. In order to identify ideal lipofection reagents in a HTS, we have developed an automated lipofection method for the transfer of reporter genes into cells and for determination of the lipofection results. The method has specifically been designed and optimized for 96-well microtiter plates and can successfully be carried out by a pipetting robot with accessory equipment. It consists of two separate parts: (1) pretransfection (preparation of liposomes, formation of lipoplexes, and lipoplex transfer to the cells) and (2) posttransfection (determination of the reporter enzyme activity and the protein content of the transfected cells). Individual steps of the lipofection method were specifically optimized - for example, lipoplex formation and incubation time as well as cell lysis, cell cultivating, and the reporter gene assay. The HTS method facilitates characterization of the transfection properties (efficiency and cytotoxicity) of large numbers of (cationic) lipids in various adherent cell types.

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