Aims and Methods A pot experiment was carried out to determine the effect of Angelica sinensis intercropped with garlic on A. sinensis growth and soil microbial communities in rhizosphere soil of A. sinensis under the scheme of A. sinensis intercropped with garlic. Results Soil microbial community was changed and bacteria functional group diversity was increased, and A. sinensis growth was improved when A. sinensis intercropped with garlic under the continuous cropping condition. Also, it shows that intercropping significantly increased root dry weight, and much less substantially shoot dry weight and plant height. Relative to the control, the activity of superoxide dismutase, peroxidase and catalase was increased by 80.00, 243.41 and 37.13%, respectively, but the content of malondialdehyde was decreased by 11.67%, along with a 70.13% yield improvement of A. sinensis, and 50.00% and 10.20% essential oil and alcohol-soluble extract increasing, respectively. The result also showed that the population of bacteria, aerobic cellulose-decomposing bacteria, organic phosphorus-solubilizing bacteria, inorganic phosphorus-solubilizing bacteria and potassium-solubilizing bacteria in rhizosphere soil were promoted in intercropping in vigorous growth stage. The abundance of aerobic cellulose-decomposing bacteria, organic phosphorussolubilizing bacteria and potassium-solubilizing bacteria in intercropping soils changed at the rootstock thickening. Although the total population of functional groups in intercropping A. sinensis rhizosphere soils was lower than in monocroping system, community diversity and evenness increased and dominance concentration decreased. Conclusions Intercropping A. sinensis with garlic can alleviate the soil sickness of A. sinensis.