AB STRACT: A suspended growth photobioreactor was utilized to treat pharmaceutical wastewater by a wild strain purple non-sulfur photosynthetic bacterium isolated from the soil. The strain was named Z08 and identified as Rhodobacter-sphaeroides by 16SrDN. The photobioreactor was illuminated externally with two (40 W) fluorescent compact light sources on both sides. Its operation pH and temperature were between 6.8 -7.0 and 20 -30 ºC, respectively. Optimum growth of the isolate was obtained after enrichment of the pharmaceutical wastewater with 0.5 % ammonium sulfate and 0.1 % yeast extract under microaerobic optimum light (6000 lx) condition at 5d retention. Using these optimum conditions, the maximum dry cell weight and chemical oxygen demand percentage removal were 880 mg/L and 80 %. Chemical analysis of the culture after treatment of the enriched and non-enriched wastewater showed the crude protein content of the biomass to be 54.6 % and 38.0 %, respectively. This study proved that photosynthetic bacteria could transform complex wastewater that contains recalcitrant organic compounds with a resultant recovery of useful products.

ABSTRACT:A new photosynthetic bacterium isolate was morphologically identified as a non-motile rod-shape gram-negative bacterium. It produced a dark red culture under phototrophic condition, reproduced by budding and formed a lamellar intracytoplasmic membrane system parallel to cytoplasmic membrane, which contained bacteriochlorophyll a and caratenoids. It's physiological and nutrient requirement tests gave indication that the isolate thrived and multiplied in varied environmental conditions. It was consequently named Z08 and identified as Rhodobacter sphaeroides by 16SrDNA. Adaptation of Z08 to biodegradation of two environmentally concerned wastewaters, i.e. soybean and pharmaceutical wastewaters, attested its potential in wastewater bioremediation. Z08 adaptation in a suspended batch photobioreactor treating pharmaceutical wastewater at 3500lx radiation recorded best result after wastewater dilution of 1:4 with concomitant chemical oxygen demand reduction, biomass yield and specific growth of 50 %, 780 mg/L and 0.015/h, respectively at the lowest hydraulic retention time of three days. Furthermore, gas chromatography mass spectra analyses of treated and raw pharmaceutical wastewater indicated that high molecular weight recalcitrant compounds found in the pharmaceutical wastewater were transformed to less toxic and acceptable lower molecular weight substances through biodegradation. Whilst Z08 treatment of soybean wastewater under natural light intensity radiation recorded 80 % reduction, 1540 mg/L and 0.025/h for chemical oxygen demand, biomass and specific growth rate respectively regardless of the food to microorganism ratio. This preliminary investigation showed that isolate Z08 has some toxic tolerance level which could detoxify refractory substances with great potential for cell protein recovery in high organic strength wastewater. Therefore, strain Z08 could be employed in biodegradation of contaminated wastewater streams.

One of the main reasons for the continued increase in the deficit of affordable housing in Nigeria is the high cost of brick made from sand and ordinary Portland cement (OPC). Finding a replacement for cement to assure sustainability is crucial as the raw materials used in making cements which are naturally occurring are depleting. Rice husk was milled and sieved to reduce the size to 0.020 mm. The moisture content of the rice husk was 9.98%, bulk density was 609.61g cm -3 and the ash content was 18.74%. Rice husk was wetted for 3, 6, 9, 12, 15 and 21 h and mixed with slaked lime at the ratios of 1:1, 3:1, 2:3 and 3:2. The composite was molded in cylindrical and rectangular mold using mechanical and hydraulic press. The compressive strength (CS) (N/mm 2 ) was tested at ages of 7, 14 and 21 days. The results show higher compressive strength in 1:1 as the bricks ages and also greater strength when wetted for 15 h. For 15 h wetting, 1:1 had CS (N/mm 2 ) of 2.59, 6.07 and 11.23. If well optimized, rice husk can be an excellent material for brick production thereby presenting a good alternative to OPC.

Laboratory simulated brewery wastewater with chemical oxygen demand (COD) and total phosphorus (TP) of 10,000 and 685.6mg/L was used to study the effects of metal ions supplement on pollutants biodegradation by phototrophic bacteria named Rhodobacter sphaeroides Z08. The results showed that the process, when operated microaerobically at 20-30°C, influent supplemented with ferrous 50mg/L, molybdenum 200mg/L, and magnesium 400mg/L yielded 55 & 38; 43 &36; 41 & 36% reductions of COD and TP respectively at three day hydraulic retention time. Binary addition of ferrous and molybdenum increased the cell growth without any significant effect on the pollutants removal. Best impact was binary addition of ferrous and magnesium which yielded still 55% COD reduction but with higher bacterial biomass (1536mg/L DCW). Chemical analysis of the treated wastewater and the harvested biomass showed that majority of the inorganic constituents of the wastewater were assimilated by the bacteria which is an indication of its metal tolerance ability.