In this study, the cytokine response (interleukin-6; IL-6), free oxygen radicals which are claimed to be responsible for the damage in the kidney tissue of exercise-trained rats and untrained-rats, and antioxidant levels were investigated after being forced to an exhausting run. Forty male Wistar albino rats were assigned to the following groups: sedentary controls (C); untrained animals that acutely completed the exhaustive exercise and were sacrificed immediately after exhaustion (UT-i) or 1 day after exhaustion (UT-1); and long-term trained animals that completed the exhaustive exercise and were sacrificed immediately after exhaustion (T-i) or 1 day after exhaustion (T-1). In UT-i and 1 day after exhaustion (T-1) groups, total oxidant status levels were increased compared to controls (P<0.05). IL-6, which is reported to have an anti-inflammatory effect in exercise, did not increase in untrained group immediately, but started to increase 1 day after exhaustion compared to controls. IL-6 levels were significantly increased in the T-i and T-1 groups compared to the control and UT-i groups (P<0.05). The level of total antioxidant status did not show a significant increase in the UT-i group but started to rise after exhaustion the T-1 group. IL-6 levels were significantly increased in the T-i and T-1 groups compared to the control, UT-i, and UT-1 groups (P<0.05). As a result, while oxidant stress and antioxidant mechanism increased immediately in the trained group, IL-6 increased significantly immediately and 1 day later. In the untrained group, however, an increase was observed in oxidant stress, antioxidant mechanism, and IL-6 levels after 1 day.