Objective: The object of the study was to examine the major pharmacokinetic parameters after a single application of a complex drug preparation for veterinary use based on fipronil, praziquantel, moxidectin, and pyriproxyfen in cats and dogs. Materials and Methods: For dogs, the drug preparation was administered spot-on solution in the following dosage of active pharmaceutical substances: fipronil 27.0 mg/kg body weight (bwt), praziquantel 10.8 mg/kg bwt, moxidectin 6.75 mg/kg bwt, and pyriproxyfen 5.4 mg/kg bwt; for cats, the dosage was the following: fipronil 43.2 mg/kg bwt, praziquantel 17.28 mg/kg bwt, moxidectin 4.32 mg/kg bwt, and pyriproxyfen 8.64 mg/kg bwt. The blood samples were taken from dogs and cats. The principle of the method for determining praziquantel, trans-4-hydroxypraziquantel, pyriproxyfen, and fipronil in serum samples was chromatographed in a high-pressure liquid chromatograph with detection by means of a mass-spectrometric detector. The moxidectin content of the blood was detected by high-performance liquid chromatography. Results: The drug preparation active substances: praziquantel, fipronil, and moxidectin are absorbed into the blood of dogs and cats. The penetration of praziquantel into the systemic circulation and further into organs and tissues was proved. After topical administration, moxidectin is absorbed and distributed systemically and is slowly removed from the plasma, which manifests itself in detectable concentrations of moxidectin in the blood for 1 month. Conclusion: The present results of pharmacokinetic investigations may promote to the determination of effective therapy strategy and prophylaxis of parasitic diseases in dogs and cats.

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The purpose of the research: toxicological evaluation of a medicinal product for veterinary use "Neoterica Protecto 12" on laboratory animals. Materials and methods. White outbred rats, mice and guinea pigs were used in the experiment. In the study of acute oral toxicity in mice and rats, acute cutaneous toxicity, subacute toxicity, irritant action of an aqueous suspension of a combination of active substances, allergenic properties of the collar «Neoterika Protecto 12» conventional methods were used. Results and discussion. The median lethal doses LD50 for oral supplementation to white mice (1070 mg/kg) and oral supplementation to white rats (3210 mg/kg) were diagnosed, which allowed to designate the preparation to the 3rd hazard class (moderately hazardous substances). In the study of acute cutaneous toxicity on white rats LD50 amounted to more than 10 000 mg/kg, therefore, according to the generally accepted hygienic classification, the preparation belongs to the 4th hazard class during cutaneous application. In the study of subacute toxicity of the medication on rats for 6 months after application of the aqueous suspension of the combination of active ingredients in doses of 1000, 500 and 200 mg/kg, no changes in general state and behavior were observed in animals. When an aqueous suspension of a combination of active substances of the collar was applied to the skin of rats, there were no signs of irritant effect, but a mild effect on the mucous membrane of the guinea pig's eyes was recorded. It was found that the preparation does not have allergic and sensitizing effects.

The purpose of the research is to study the acute oral toxicity of the medicinal product for veterinary use Iverbutan, intended for the treatment and prevention of arachnoentomoses and nematodoses of poultry.Materials and methods. The studies were carried out on 30 outbred male rats weighing 210-240 g and 60 mice weighing 18–21 g. The animals were divided into experimental and control groups. The drug was administered once without dilution in the form of the provided solution using an intragastric tube. Doses of 2000, 4000, 6000, 8000 and 10 000 mg/kg were tested on mice, and on rats – 10 000, 8000, 5000, 4000 mg/kg. The animals of the control groups were injected with drinking water. Within 14 days after a single dose of the drug, the physiological state and behavior of animals, possible death, as well as the manifestation of symptoms of intoxication were monitored. The control of the body weight of the animals of the experimental and control groups was carried out on the day of the experiment (before drug administration), as well as on the 1st, 3rd, 7th, 9th and 14th days.Results and discussion. It was found that after oral administration of iverbutan to experimental animals, the average lethal dose, calculated by the Kerber method, was 5600 mg/kg of body weight in mice and 7000 mg/kg of body weight in rats (hazard class 4 according to GOST 12.1.007-76). The average lethal dose, calculated by the Miller and Tainter method, was 5292.0±1058.6 (4233.4÷6350.6) mg/kg of body weight in mice and 6463.2±1496.9 (4966.3÷7960.1) mg/kg of body weight of rats (hazard class 3 according to GOST 12.1.007-76), which indicates species sensitivity.

Since 2014, agricultural and medical scientific institutions, previously been a part of the specialized agricultural and medical academies of sciences are under the scientific and methodological guidance of the Russian Academy of Sciences (RAS). Among them is the All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plants – a branch of the Federal State Budget Scientific Institution “Federal Scientific Centre VIEV” (VNIIP – FSC VIEV), named after K. I. Skryabin and Ya. R. Kovalenko.Special attention in its scientific research is devoted to the study of formation and functioning patterns of parasitic systems; to the development of theoretical foundations to prevent parasitic diseases in livestock animals and plants, and to the improvement and implementation of integrated systems for protecting humans, animals and plants from socially dangerous and economically significant parasitic diseases by creating new methods for early diagnosis and prevention based on the widespread use of biotechnology, genetics and immunology; and to invention of fundamentally new means of protecting animals and plants that provide a high economic effect.The implementation of the Institute’s main tasks predetermines active scientific and publishing activities that can be designated as the final stage of any research work; therefore, scientific and research institutions publish monographs, collections of scientific papers, reference books, study guides, etc. The purpose of the article is to present main directions of VNIIP’s research and publishing activities highlighting features that characterize certain types of publications.The characteristic is given for two printed publications on advanced scientific research in parasitology, namely, the collection of materials from the “Theory and Practice of Parasitic Disease Control” Conference and the Russian Journal of Parasitology. A typological analysis of publications is given, and its results are presented. The declared features of the publications confirm their quality and meet key requirements of the current book publishing industry in this direction.The material is of interest to promote achievements and findings of the most important studies conducted by the team of scientists of the Institute and will be useful both to publishers and to authors of publications.