Purpose: Hemorrhagic cystitis (HC) is the most common urotoxic side effect of cyclophosphamide (CYP). Platelet rich plasma (PRP) plays an important role in wound healing and inflammatory responses. The aim of this study was to investigate the efficacy of intravesical PRP at treatment of interstitial cystitis (IC). Methods: Female rats (n=24) were used. IC was induced by intraperitoneal injection of cyclophosphamide (CYP) and rats were randomly allocated to one of four groups (n = 6 per group): A control group; a sham group with saline (75 mg/kg; i.p.) instead of CYP on day 1, IC group, which was injected with CYP (150 mg/kg; i.p.) on day 1; and, an intravesical PRP‑treated group which was injected with CYP (150 mg/kg; i.p.) on day 1. On day 2, the rats in each group were sacrificed under anesthesia. Results: Histological evaluation showed CYP administration induced severe IC with marked edema, hemorrhage and inflammation in CYP and CYP+PRP groups, but that PRP did not suppress these histopathological changes. Conclusion: PRP did not suppress the histopathological changes in rats that had IC due to cyclophosphamide injection.