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A reusable water bottle was swabbed as part of the citizen science project “Swab and Send,” and a Klebsiella grimontii isolate was recovered on chromogenic agar and designated SS141. Whole‐genome sequencing of SS141 showed it has the potential to be a human pathogen as it contains the biosynthetic gene cluster for the potent cytotoxin, kleboxymycin, and genes for other virulence factors. The genome also contains the antibiotic‐resistant genes, blaOXY‐6‐4, and a variant of fosA, which is likely to explain the observed resistance to ampicillin, amoxicillin, and fosfomycin. We have also shown that SS141 forms biofilms on both polystyrene and polypropylene surfaces, providing a reasonable explanation for its ability to colonize a reusable water bottle. With the increasing use of reusable water bottles as an alternative to disposables and a strong forecast for growth in this industry over the next decade, this study highlights the need for cleanliness comparable to other reusable culinary items.

SUMMARYDog faeces collected from a public park were cultured on selective media for Campylobacter spp. Campylobacter jejuni was isolated from 12 (4-6 %) of 260 specimens. In contrast Salmonella spp. were found in only three (12 %). Six of the 12 isolates were nalidixic acid-resistant thermophilic campylobacters (NARTC), whereas during the same period of study none were found among human isolates. Most of the campylobacter positive faeces were found during June and July. Dog faeces deposited in public places constitute only a small potential source of infection by this organism.

SUMMARYLiving flies collected from three locations were cultured on selective medium for Campylobacter spp. Campylobacter jejuni was isolated from five (2-4 00) of 210 flies examined.These results suggest that the potential hazard to health from the transmission of campylobacters from animals to human food by flies is small.

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